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    Analysis of the Role of Bacillus oleronius Proteins in the Induction of Rosacea and Evaluation of Novel Therapeutics for the Treatment of Dermal and Ocular Rosacea


    Maher, Amie (2017) Analysis of the Role of Bacillus oleronius Proteins in the Induction of Rosacea and Evaluation of Novel Therapeutics for the Treatment of Dermal and Ocular Rosacea. PhD thesis, National University of Ireland Maynooth.

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    Abstract

    Rosacea is a chronic inflammatory condition predominantly affecting the face on the cheeks, forehead, nose, chin and in some cases eyes. The primary diagnostic feature of rosacea is persistent erythema on the central region of the face. Distinct secondary features help to diagnose one of the four subtypes of rosacea and commonly include telangiectasia, flushing, papule and pustules, thickening of the skin and oedema. Rosacea is a debilitating condition that can impact on a patients emotional and psychological state. The aetiology of this dermatological condition is unknown and multiple factors contribute to the onset of rosacea and exacerbate symptoms. Exogenous factors such as radiation or diet, and endogenous factors such as sebum alteration or stress, can change skin homeostasis. These factors can influence resident microbiota, which may alter skin homeostasis and induce the production of virulence factors. The increased density of Demodex mites in rosacea patients has been demonstrated previously. Demodex mites cause micro-abrasions to the skin surface and weaken the skin barrier function. Bacillus oleronius isolated from Demodex is a bacterium associated with rosacea pathogenesis and patient serum previously exposed to antigen have displayed immune reactivity. The work in this thesis investigated the effect of different stress conditions on B. oleronius antigen production. The proteome of B. oleronius was assessed in response to temperature stress and oxidative stress to evaluate differential abundance in protein production. B. oleronius proteins may contribute to the over-activation of the innate immune response, leading to increased gene expression of cytokines, increased tissue damage and onset of chronic inflammation. The treatment and management of rosacea is targeted towards symptomatic relief, predominantly with the aid of antibiotics. Some treatments demonstrate antibacterial and anti-inflammatory effects, both of which help to treat patient symptoms. Three blocking agents were evaluated in this research to determine the potential capturing of B. oleronius antigens. Mucin is naturally present in tear fluid and protects the epithelial barrier. This antimicrobial displayed potential as an adhesive decoy against antigen. A novel saline therapeutic was also investigated to treat rosacea patients and alleviate symptoms. The novel salt based formulation demonstrated effective anti-inflammatory properties in two pilot studies. There is no cure for rosacea, however the work presented here demonstrated potential therapeutics for rosacea patient treatment against B. oleronius antigens.

    Item Type: Thesis (PhD)
    Keywords: Analysis; Bacillus oleronius Proteins; Induction; Rosacea; Evaluation; Novel Therapeutics; Treatment; Dermal and Ocular Rosacea;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 10036
    Depositing User: IR eTheses
    Date Deposited: 02 Oct 2018 11:21
    URI:

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