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    Retracing the in vivo haematopoietic tree using single‐cell methods


    Perie, Leila and Duffy, Ken R. (2016) Retracing the in vivo haematopoietic tree using single‐cell methods. FEBS Letters, 590 (22). pp. 4068-4083. ISSN 0014-5793

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    Abstract

    The dynamic process by which self‐renewing stem cells and their offspring proliferate and differentiate to create the erythroid, myeloid and lymphoid lineages of the blood system has long since been an important topic of study. A range of recent single cell and family tracing methodologies such as massively parallel single‐cell RNA‐sequencing, mass cytometry, integration site barcoding, cellular barcoding and transposon barcoding are enabling unprecedented analysis, dissection and re‐evaluation of the haematopoietic tree. In addition to the substantial experimental advances, these new techniques have required significant theoretical development in order to make biological deductions from their data. Here, we review these approaches from both an experimental and inferential point of view, considering their discoveries to date, their capabilities, limitations and opportunities for further development.

    Item Type: Article
    Additional Information: This is the preprint version of the published article, which is available at https://doi.org/10.1002/1873-3468.12299
    Keywords: hematopoietic tree; lineage tracing; single cells; RNA sequencing; mass cytometry; barcoding;
    Academic Unit: Faculty of Science and Engineering > Research Institutes > Hamilton Institute
    Item ID: 10173
    Identification Number: https://doi.org/10.1002/1873-3468.12299
    Depositing User: Dr Ken Duffy
    Date Deposited: 02 Nov 2018 17:51
    Journal or Publication Title: FEBS Letters
    Publisher: Wiley
    Refereed: Yes
    Funders: Science Foundation Ireland (SFI), CNRS, Bettencourt‐Schueller Foundation, Labex CelTisPhyBio, Idex Paris‐Science‐Lettres Program
    URI:

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