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    In vitro development and in vivo application of a platinum-based electrochemical device for continuous measurements of peripheral tissue oxygen


    Finnerty, Niall J. and Bolger, Fiachra B. (2018) In vitro development and in vivo application of a platinum-based electrochemical device for continuous measurements of peripheral tissue oxygen. Bioelectrochemistry, 119. pp. 124-135. ISSN 1567-5394

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    Abstract

    Acute limb ischaemia is caused by compromised tissue perfusion and requires immediate attention to reduce the occurrence of secondary complications that could lead to amputation or death. To address this, we have developed a novel platinum (Pt)-based electrochemical oxygen (O2) device for future applications in clinical monitoring of peripheral tissue ischaemia. The effect of integrating a Pt pseudo-reference electrode into the O2 device was investigated in vitro with an optimum reduction potential of −0.80 V. A non-significant (p = 0.11) decrease in sensitivity was recorded when compared against an established Pt-based O2 sensor operating at −0.65 V. Furthermore, a biocompatible clinical sensor (ClinOX) was designed, demonstrating excellent linearity (R2 = 0.99) and sensitivity (1.41 ± 0.02 nA μM−1 ) for O2 detection. Significant rapid decreases in the O2 current during in vivo ischaemic insults in rodent limbs were reported for Pt-Pt (p b 0.001) and ClinOX (p b 0.01) and for ClinOX (p b 0.001) in porcine limbs. Ex vivo sensocompatibility investigations identified no significant difference (p = 0.08) in sensitivity values over 14 days of exposure to tissue homogenate. The Pt-Pt based O2 design demonstrated high sensitivity for tissue ischaemia detection and thus warrants future clinical investigation.

    Item Type: Article
    Keywords: Electrochemical; Tissue oxygen; Platinum; Ischaemia; Reperfusion; In vivo; Clinical;
    Academic Unit: Faculty of Science and Engineering > Chemistry
    Item ID: 13105
    Identification Number: https://doi.org/10.1016/j.bioelechem.2017.09.010
    Depositing User: Niall Finnerty
    Date Deposited: 25 Jun 2020 16:13
    Journal or Publication Title: Bioelectrochemistry
    Publisher: Elsevier
    Refereed: Yes
    URI:
    Use Licence: This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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