Murphy, Sandra and Ohlendieck, Kay
(2018)
Proteomic profiling of large myofibrillar proteins from dried and long-term
stored polyacrylamide gels.
Analytical Biochemistry, 543.
pp. 8-11.
ISSN 0003-2697
Abstract
A method for the utilization of dried polyacrylamide gels from the pre-proteomic era is described in order to
enable the mass spectrometric analysis of long-term stored protein preparations. The in-gel digestion of highmolecular-mass proteins embedded in a 20-year old gel was carried out following gel re-swelling and resulted in
the proteomic identification of a large number of proteins, including 3400 kDa titin, 800 kDa nebulin and
myosin heavy chains of 220 kDa from rabbit skeletal muscle. These findings demonstrate that dried protein gels
from past biochemical analyses can be successfully reused and analyzed by modern and refined mass spectrometric techniques.
| Item Type: |
Article
|
| Keywords: |
Gel drying;
Gel electrophoresis;
In-gel digestion;
Mass spectrometry;
Proteomics;
Skeletal muscle; |
| Academic Unit: |
Faculty of Science and Engineering > Biology |
| Item ID: |
13137 |
| Identification Number: |
https://doi.org/10.1016/j.ab.2017.11.022 |
| Depositing User: |
Prof. Kay Ohlendieck
|
| Date Deposited: |
22 Jul 2020 15:17 |
| Journal or Publication Title: |
Analytical Biochemistry |
| Publisher: |
Elsevier Masson |
| Refereed: |
Yes |
| Funders: |
Hume scholarship (Maynooth University), Muscular Dystrophy Ireland, Health Research Board (HRB), Science Foundation Ireland (SFI) |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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