MURAL - Maynooth University Research Archive Library



    Supporting Information: Fast, Copper-Free Click Chemistry, A Convenient Solid-Phase Approach to Oligonucleotide Conjugation


    Singh, Ishwar and Vyle, Joseph S. and Heaney, Frances (2009) Supporting Information: Fast, Copper-Free Click Chemistry, A Convenient Solid-Phase Approach to Oligonucleotide Conjugation. Documentation. Royal Society of Chemistry.

    [img] Download (1MB)


    Share your research

    Twitter Facebook LinkedIn GooglePlus Email more...



    Add this article to your Mendeley library


    Abstract

    General experimental Analytical TLC was performed on precoated (250 μm) silica gel 60 F-254 plates from Merck. All plates were visualized by UV irradiation, and/or staining with 5% H2SO4 in ethanol followed by heating. Flash chromatography grade silica gel 60 (230-400 mesh) was obtained from Merck. Mass analysis was performed on an Ettan MALDI-TOF Pro from Amersham Biosciences or LASER-TOF LT3 from Scientific Analytical Instruments with 3- hydroxypicolinic acid or 2,’ 4’, 6’-trihydroxyacetophenone as matrix. The NMR spectra were obtained at 1H (300 MHz), 13C (75 MHz) and 31P (121 MHz) on a Bruker instrument at 25 ºC. Chemical shifts are reported in ppm downfield from TMS as standard. HPLC was carried out using a Gilson instrument equipped with a UV detector and a Nucleosil C18 column (4.0 × 250 mm) or Phenomenex Clarity. Fluorescence spectra were recorded on a Varian Cary Eclipse instrument. All other chemical agents were purchased from Aldrich Chemical Company unless otherwise noted.

    Item Type: Monograph (Documentation)
    Additional Information: (c) The Royal Society of Chemistry 2009
    Keywords: Copper-Free Click Chemistry; Solid-Phase Approach; Oligonucleotide Conjugation;
    Academic Unit: Faculty of Science and Engineering > Computer Science
    Item ID: 2707
    Depositing User: Dr. Frances Heaney
    Date Deposited: 14 Sep 2011 13:52
    Publisher: Royal Society of Chemistry
    URI:

    Repository Staff Only(login required)

    View Item Item control page

    Downloads

    Downloads per month over past year