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    Diverse populations of T cells with NK cell receptors accumulate in the human intestine in health and in colorectal cancer


    O'Keeffe, Joan and Doherty, Derek G. and Kenna, Tony and Sheahan, Kieran and O'Donoghue, Diarmuid and Hyland, John M. and O'Farrelly, Cliona (2004) Diverse populations of T cells with NK cell receptors accumulate in the human intestine in health and in colorectal cancer. European Journal of Immunology, 34. pp. 2110-2119.

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    Abstract

    T cells expressing NK cell receptors (NKR) display rapid MHC-unrestricted cytotoxicity and potent cytokine secretion and are thought to play roles in immunity against tumors. We have quantified and characterized NKR+ T cells freshly isolated from epithelial and lamina propria layers of duodenum and colon from 16 individuals with no evidence of gastrointestinal disease and from tumor and uninvolved tissue from 19 patients with colorectal cancer. NKR+ T cell subpopulations were differentially distributed in different intestinal compartments, and CD161+ T cells accounted for over one half of T cells at all locations tested. Most intestinal CD161+ T cells expressed § g TCR and either CD4 or CD8. Significant proportions expressed HLA-DR, CD69 and Fas ligand. Upon stimulation in vitro, CD161+ T cells produced IFN- + and TNF- § but not IL-4. NKT cells expressing the V § 24V g 11 TCR, which recognizes CD1d, were virtually absent from the intestine, but colonic cells produced IFN- + in response to the NKT cell agonist ligand § -galactosylceramide. NKR+ T cells were not expanded in colonic tumors compared to adjacent uninvolved tissue. The predominance, heterogeneity and differential distribution of NKR+ T cells at different intestinal locations suggests that they are central to ntestinal immunity.

    Item Type: Article
    Keywords: Human / Intestine / T lymphocytes / NKT cells / Tumor immunity
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 328
    Depositing User: Dr. Derek Doherty
    Date Deposited: 23 Sep 2008
    Journal or Publication Title: European Journal of Immunology
    Publisher: WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
    Refereed: Yes
    URI:

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