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    Altered Activity of Peroxidase and Oxalate Oxidase Influences Lignification in Transgenic Tobacco


    Burbridge, Emma and Kjærsgaard Rasmussen, Søren and Bernier, Francois and Kaare Kristensen, Brian and McCabe, Paul Francis and Dix, Philip (2014) Altered Activity of Peroxidase and Oxalate Oxidase Influences Lignification in Transgenic Tobacco. The Open Plant Science Journal, 2014, 8, 1-8, 8. pp. 1-8. ISSN 1874-2947

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    Abstract

    Peroxidase and hydrogen peroxide both play important roles in the final stages of the lignification pathway. Peroxidase, in the presence of H2O2 catalyses the oxidation of monolignols to give lignin. In order to examine this process we looked at lignification in transgenic tobacco plants expressing a barley peroxidase gene, HvPrx8, either alone or in combination with a wheat germin gene, g.f 2.8, which encodes oxalate oxidase, thereby providing a source of H2O2. Elevated activity of the antioxidant ascorbate peroxidase was found in plants expressing oxalate peroxidase and was greatly increased by co-expression with the barley peroxidase, although the latter had no effect when expressed alone. An increase was observed in the oxidation of the lignin monomer, syringaldazine in cell lines over-expressing barley peroxidase, while a decrease was observed in double transformants. Plants over-expressing barley peroxidase have elevated levels of lignin deposition compared to that of wild type tobacco plants. Over-expression of the individual enzymes was also shown to enhance heat-induced programmed cell death (PCD) in cell suspension cultures, an effect which was greatly reduced in the double-expressing lines.

    Item Type: Article
    Additional Information: Published under the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/legalcode ) which permits unrestricted, non-commercial use, archiving, distribution and reproduction in any medium, provided that the work is properly cited. The original version of this article is available at DOI: 10.2174/1874294720140624001 .
    Keywords: Lignin; oxalate oxidase; peroxidase; syringaldazine;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 5626
    Identification Number: https://doi.org/10.2174/1874294720140624001
    Depositing User: Prof. Philip J. Dix
    Date Deposited: 16 Dec 2014 17:08
    Journal or Publication Title: The Open Plant Science Journal, 2014, 8, 1-8
    Publisher: Bentham Open Access
    Refereed: Yes
    URI:

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