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    An analysis of the phosphoproteome of immune cell lines exposed to the immunomodulatory mycotoxin deoxynivalenol


    Nogueira da Costa, Andre and Kenn, Jeffrey N. and Wild, Christopher P. and Findlay, John B. C. (2011) An analysis of the phosphoproteome of immune cell lines exposed to the immunomodulatory mycotoxin deoxynivalenol. Biochimica et Biophysica Acta - Proteins and Proteomics, 1814 (7). pp. 850-857. ISSN 1570-9639

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    Abstract

    The mycotoxin deoxynivalenol (DON) commonly contaminates cereal grains. It is ubiquitous in the Western European diet, although chronic, low-dose effects in humans are not well described, but immunotoxicity has been reported. In this study, two-dimensional gel electrophoresis was used to identify phosphoproteomic changes in human B (RPMI1788) and T (Jurkat E6.1) lymphocyte cell lines after exposure to modest concentrations of DON (up to 500 ng/mL) for 24 h. Proteins identified as having altered phosphorylation state post-treatment (C-1-tetrahydrofolate synthase, eukaryotic elongation factor 2, nucleoside diphosphate kinase A, heat shock cognate 71 kDa protein, eukaryotic translation initiation factor 3 subunit I and growth factor receptor-bound protein 2) are involved in regulation of metabolic pathways, protein biosynthesis and signaling transduction. All exhibited a greater than 1.4-fold change, reproducible in three separate experiments consisting of 36 gels in total. Flow cytometry validated the observations for eukaryotic elongation factor 2 and growth factor receptor-bound protein 2. These findings provide further insights as to how low dose exposure to DON may affect human immune function and may have potential as mechanismbased phosphoprotein biomarkers for DON exposure.

    Item Type: Article
    Additional Information: The definitive version of this article is available at doi:10.1016/j.bbapap.2011.04.001
    Keywords: Biomarker; Deoxynivalenol; Mycotoxin; Phosphoproteomics;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 5994
    Identification Number: https://doi.org/10.1016/j.bbapap.2011.04.001
    Depositing User: Professor John Findlay
    Date Deposited: 31 Mar 2015 14:47
    Journal or Publication Title: Biochimica et Biophysica Acta - Proteins and Proteomics
    Publisher: Elsevier
    Refereed: Yes
    URI:

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