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    Complete Chloroplast Genome Sequence of a Major Allogamous Forage Species, Perennial Ryegrass (Lolium perenne L.)


    Diekmann, Kerstin and Hodkinson, Trevor R. and Wolfe, Kenneth H. and van den Bekerom, Rob Jozef Matheus and Dix, Philip and Barth, Susanne (2009) Complete Chloroplast Genome Sequence of a Major Allogamous Forage Species, Perennial Ryegrass (Lolium perenne L.). DNA Research, 16. pp. 165-176. ISSN 1340-2838

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    Abstract

    Lolium perenne L. (perennial ryegrass) is globally one of the most important forage and grassland crops. We sequenced the chloroplast (cp) genome of Lolium perenne cultivar Cashel. The L. perenne cp genome is 135 282 bp with a typical quadripartite structure. It contains genes for 76 unique proteins, 30 tRNAs and four rRNAs. As in other grasses, the genes accD, ycf1 and ycf2 are absent. The genome is of average size within its subfamily Pooideae and of medium size within the Poaceae. Genome size differences are mainly due to length variations in non-coding regions. However, considerable length differences of 1–27 codons in comparison of L. perenne to other Poaceae and 1–68 codons among all Poaceae were also detected. Within the cp genome of this outcrossing cultivar, 10 insertion/deletion polymorphisms and 40 single nucleotide polymorphisms were detected. Two of the polymorphisms involve tiny inversions within hairpin structures. By comparing the genome sequence with RT–PCR products of transcripts for 33 genes, 31 mRNA editing sites were identified, five of them unique to Lolium. The cp genome sequence of L. perenne is available under Accession number AM777385 at the European Molecular Biology Laboratory, National Center for Biotechnology Information and DNA DataBank of Japan.

    Item Type: Article
    Additional Information: The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org
    Keywords: chloroplast genome; Lolium perenne; Poaceae; chloroplast DNA variation; RNA editing;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 6258
    Identification Number: https://doi.org/10.1093/dnares/dsp008
    Depositing User: Prof. Philip J. Dix
    Date Deposited: 15 Jul 2015 14:21
    Journal or Publication Title: DNA Research
    Publisher: Oxford University Press
    Refereed: Yes
    Funders: Science Foundation Ireland (SFI), Teagasc
    URI:

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