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    Unexpected DNA binding properties with correlated downstream biological applications in mono vs. bis-1,8-naphthalimide Ru(II)-polypyridyl conjugates


    Ryan, Gary J. and Poynton, Fergus E. and Elmes, Robert B.P. and Erby, Marialuisa and Williams, D. Clive and Quinn, Susan J. and Gunnlaugsson, Thorfinnur (2015) Unexpected DNA binding properties with correlated downstream biological applications in mono vs. bis-1,8-naphthalimide Ru(II)-polypyridyl conjugates. Dalton Transactions, 44. pp. 16332-16344. ISSN 1477-9226

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    Abstract

    The synthesis, spectroscopic characterisation and biological evaluation of mono- and bis-1,8-naphthalimide- conjugated ruthenium(II)-polypyridyl complexes is presented. Spectroscopic DNA titrations, together with denaturation studies, show strong binding of both species to DNA through the naphthalimide arms. Linear and circular dichroism (LD and CD) spectroscopy reveal close association of the Ru(bpy)3 2+ core with DNA in the case of the mono-naphthalamide complex, [Ru(bpy)2(bpy-NAP)]2+. Significantly, binding by the second naphthalimide arm in the [Ru(bpy)2(bpy-NAP2)]2+ complex is found to displace the Ru(bpy)3 2+ centre from the DNA backbone. This ‘negative allosteric effect’ is found to have a dramatic influence on the photoinduced damage of plasmid DNA, and the viability of HeLa cancer cells upon photoactivation. Overall the study clearly maps and correlates the relationship between molecular structure, in vitro binding and activity, and in cellulo function.

    Item Type: Article
    Keywords: DNA; binding properties; correlated; downstream; biological applications; mono vs. bis-1,8-naphthalimide Ru(II)-polypyridyl conjugates;
    Academic Unit: Faculty of Science and Engineering > Chemistry
    Item ID: 6485
    Identification Number: https://doi.org/10.1039/c5dt00360a
    Depositing User: Robert Elmes
    Date Deposited: 20 Oct 2015 13:33
    Journal or Publication Title: Dalton Transactions
    Publisher: Royal Society of Chemistry
    Refereed: Yes
    URI:

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