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    Antioxidant defence in UV-irradiated tobacco leaves is centred on hydrogen-peroxide neutralization


    Majer, Petra and Czegeny, Gyula and Sandor, Gyorgyi and Dix, Philip and Hideg, Eva (2014) Antioxidant defence in UV-irradiated tobacco leaves is centred on hydrogen-peroxide neutralization. Plant Physiology and Biochemistry, 82. pp. 239-243. ISSN 0981-9428

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    Official URL: http://dx.doi.org/10.1016/j.plaphy.2014.06.011


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    Abstract

    Greenhouse grown tobacco (Nicotiana tabacum L. cv. Petit Havana) plants were exposed to supplemental UV centred at 318 nm and corresponding to 13.6 kJ m-2 d-1 biologically effective UV-B (280e315 nm) radiation. After 6 days this treatment decreased photosynthesis by 30%. Leaves responded by a large increase in UV-absorbing pigment content and antioxidant capacities. UV-stimulated defence against ROS was strongest in chloroplasts, since activities of plastid enzymes FeSOD and APX had larger relative increases than other, non-plastid specific SODs or peroxidases. In addition, non-enzymatic defence against hydroxyl radicals was doubled in UV treated leaves as compared to controls. In UV treated leaves, the extent of activation of ROS neutralizing capacities followed a peroxidases > hydroxyl-radical neutralization > SOD order. These results suggest that highly effective hydrogen peroxide neutralization is the focal point of surviving UV-inducible oxidative stress and argue against a direct signalling role of hydrogen peroxide in maintaining adaptation to UV, at least in laboratory experiments.

    Item Type: Article
    Additional Information: The definitive published version of this article is available at DOI: 10.1016/j.plaphy.2014.06.011
    Keywords: Antioxidant; Peroxidase; Photosynthesis; Reactive oxygen species; Hydrogen peroxide; Tobacco; UV treatment;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 6833
    Identification Number: https://doi.org/10.1016/j.plaphy.2014.06.011
    Depositing User: Prof. Philip J. Dix
    Date Deposited: 19 Jan 2016 10:33
    Journal or Publication Title: Plant Physiology and Biochemistry
    Publisher: Elsevier
    Refereed: Yes
    Funders: Hungarian Scientific Grant Agency
    URI:

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