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    Subproteomic analysis of basic proteins in aged skeletal muscle following offgel pre-fractionation


    Gannon, Jay and Ohlendieck, Kay (2012) Subproteomic analysis of basic proteins in aged skeletal muscle following offgel pre-fractionation. Molecular Medicine Reports, 5 (4). pp. 993-1000. ISSN 1791-2997

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    Abstract

    The progressive loss of skeletal muscle mass is a serious pathophysiological problem in the elderly, which warrants detailed biochemical studies into the underlying mechanism of age-related fiber degeneration. Over the last few years, mass spectrometry (MS)-based proteomics has identified a considerable number of new biomarkers of muscle aging in humans and animal models of sarcopenia. However, interpretation of the proteomic findings is often complicated by technical and biological limitations. Although gel electrophoresis-based approaches represent a highly sensitive analytical way for the large-scale and high-throughput survey of global changes in skeletal muscle proteins during aging, often the presence of components with an isoelectric point in the basic range is underestimated. We, therefore, carried out a comparative subproteomic study of young versus aged rat muscle focusing on potential changes in muscle proteins with an alkaline isoelectric point, using a combination of offgel electrophoresis and two-dimensional (2D) slab gel electrophoresis. Offgel electrophoresis was successfully applied as a prefractionation step to enrich basic protein species from crude tissue extracts representing young adult versus senescent muscle specimens. Proteomics has demonstrated alterations in a small cohort of basic proteins during muscle aging. The mass spectrometric identification of altered proteins and immunoblotting revealed a decrease in the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and a concomitant increase in mitochondrial creatine kinase (CK) and ubiquinol cytochrome‑c reductase. This agrees with the idea of a glycolytic-to-oxidative shift during muscle aging, which is indicative of an overall fast-to-slow transition process in senescent rat muscle. Thus, alterations in the abundance of metabolic enzymes appear to play a central role in the molecular pathogenesis of age‑dependent muscle wasting.

    Item Type: Article
    Additional Information: GANNON J, OHLENDIECK K. Subproteomic analysis of basic proteins in aged skeletal muscle following offgel pre-fractionation. Molecular Medicine Reports. 2012;5(4):993-1000. doi:10.3892/mmr.2012.759.
    Keywords: aging; muscle aging; muscle proteomics; offgel electrophoresis; sarcopenia;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 6912
    Identification Number: https://doi.org/10.3892/mmr.2012.759
    Depositing User: Prof. Kay Ohlendieck
    Date Deposited: 21 Jan 2016 14:37
    Journal or Publication Title: Molecular Medicine Reports
    Publisher: Spandidos Publications
    Refereed: Yes
    Funders: Science Foundation Ireland (SFI), Higher Education Authority (HEA)
    URI:

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