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    Close temporal coupling of neuronal activity and tissue oxygen responses in rodent whisker barrel cortex


    Li, Jennifer and Bravo, Diego S. and Upton, A. Louise and Gilmour, Gary and Tricklebank, Mark and Fillenz, Marianne and Martin, Chris and Lowry, John P. and Bannerman, David M. and McHugh, Stephen B. (2011) Close temporal coupling of neuronal activity and tissue oxygen responses in rodent whisker barrel cortex. European Journal of Neuroscience, 34. pp. 1983-1996. ISSN 0953-816X

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    Abstract

    Neuronal activity elicits metabolic and vascular responses, during which oxygen is first consumed and then supplied to the tissue via an increase in cerebral blood flow. Understanding the spatial and temporal dynamics of blood and tissue oxygen (To2 ) responses following neuronal activity is crucial for understanding the physiological basis of functional neuroimaging signals. However, our knowledge is limited because previous To2 measurements have been made at low temporal resolution (>100 ms). Here we recorded To2 at high temporal resolution (1 ms), simultaneously with co-localized field potentials, at several cortical depths from the whisker region of the somatosensory cortex in anaesthetized rats and mice. Stimulation of the whiskers produced rapid, laminar-specific changes in To2 . Positive To2 responses (i.e. increases) were observed in the superficial layers within 50 ms of stimulus onset, faster than previously reported. Negative To2 responses (i.e. decreases) were observed in the deeper layers, with maximal amplitude in layer IV, within 40 ms of stimulus onset. The amplitude of the negative, but not the positive, To2 response correlated with local field potential amplitude. Disruption of neurovascular coupling, via nitric oxide synthase inhibition, abolished positive To2 responses to whisker stimulation in the superficial layers and increased negative To2 responses in all layers. Our data show that To2 responses occur rapidly following neuronal activity and are laminar dependent.

    Item Type: Article
    Additional Information: The definitive version of this article is available at DOI: 10.1111/j.1460-9568.2011.07927.x
    Keywords: amperometry; barrel cortex; hemodynamic response; local field potential; tissue oxygen;
    Academic Unit: Faculty of Science and Engineering > Chemistry
    Item ID: 7008
    Identification Number: https://doi.org/10.1111/j.1460-9568.2011.07927.x
    Depositing User: John Lowry
    Date Deposited: 04 Mar 2016 17:14
    Journal or Publication Title: European Journal of Neuroscience
    Publisher: Blackwell Publishing
    Refereed: Yes
    URI:

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