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    Purification and Identification of a 7.6-kDa Protein in Media Conditioned by Superinvasive Cancer Cells


    Dowling, Paul and Maurya, Priyanka and Meleady, Paula and Glynn, Sharon and Dowd, Andrew and Henry, Michael and Clynes, Martin (2007) Purification and Identification of a 7.6-kDa Protein in Media Conditioned by Superinvasive Cancer Cells. Anticancer Research, 27 (3A). pp. 1309-1317. ISSN 0250-7005

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    Abstract

    Background: Selection of the human drug sensitive and invasive cell line (MDA-MB-435S-F) with the chemotherapeutic agent paclitaxel, resulted in the development of drug resistant cell lines displaying enhanced invasion-related characteristics. Materials and Methods: Serum-free conditioned media from the human cancer drug-sensitive and invasive cell line (MDA-MB-435S-F) and its paclitaxel-resistant superinvasive variant (MDA-MB-435S-F/Taxol10p4pSI) were analyzed using Surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS). Results: A differentially expressed protein was observed at 7.6 kDa, which was 4-fold upregulated in MDA-MB-435S-F/Taxol10p4pSI. The differentially expressed protein was identified using matrix-assisted laser desorption ionization tandem time-of-flight mass spectrometry (MALDI-TOF/TOF MS), as a fragment of bovine transferrin. The transferrin receptor was also found to be overexpressed in the superinvasive cell line. Conclusion: Cleavage of serum proteins such as transferrin could provide a valuable source of markers for malignant tumours and could also play a role in aspects of cancer pathogenesis, such as tumour cachexia.

    Item Type: Article
    Keywords: Drug resistant; IMAC; SELDI; superinvasive; transferrin;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 7355
    Depositing User: Paul Dowling
    Date Deposited: 18 Aug 2016 15:23
    Journal or Publication Title: Anticancer Research
    Publisher: The International Institute of Anticancer Research
    Refereed: Yes
    Funders: Higher Education Authority (HEA), Science Foundation Ireland (SFI)
    URI:

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