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    2-D difference gel electrophoresis of the lung squamous cell carcinoma versus normal sera demonstrates consistent alterations in the levels of ten specific proteins


    Dowling, Paul and O'Driscoll, Lorraine and Meleady, Paula and Henry, Michael and Roy, Shunil and Ballot, Jo and Moriarty, Michael and Crown, John and Clynes, Martin (2007) 2-D difference gel electrophoresis of the lung squamous cell carcinoma versus normal sera demonstrates consistent alterations in the levels of ten specific proteins. Electrophoresis, 28 (23). pp. 4302-4310. ISSN 0173-0835

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    Abstract

    Most lung cancers are diagnosed too late for curative treatment to be possible, therefore early detection is crucial. Serum proteins are a rich source of biomarkers and have the potential to be used as diagnostic and prognostic indicators for lung cancer. In order to examine differences in serum levels of specific proteins associated with human lung squamous carcinoma, immunodepletion of albumin and five other high-abundant serum proteins followed by 2-D difference gel electrophoresis (DIGE) analysis and subsequent MS was used to generate a panel of proteins found to be differentially expressed between the cancer and normal samples. Proteins found to have increased abundance levels in squamous cell carcinoma sera compared to normal sera included apolipoprotein A-IV precursor, chain F; human complement component C3c, haptoglobin, serum amyloid A protein precursor and Ras-related protein Rab-7b. Proteins found to have lower abundance levels in squamous cell carcinoma sera compared to normal sera included alpha-2-HS glycoprotein, hemopexin precursor, proapolipoprotein, antithrombin III and SP40; 40. The data presented here demonstrate that high-abundant protein removal combined with 2-D DIGE is a powerful strategy for the discovery of potential biomarkers. The identification of lung cancer-specific biomarkers is crucial to early detection, which in turn could lead to a dramatic increase in survival rates.

    Item Type: Article
    Keywords: Biomarker; Difference gel electrophoresis; Immunodepletion; Serum; Squamous;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 7359
    Identification Number: https://doi.org/10.1002/elps.200700246
    Depositing User: Paul Dowling
    Date Deposited: 18 Aug 2016 15:21
    Journal or Publication Title: Electrophoresis
    Publisher: Wiley-VCH Verlag
    Refereed: Yes
    Funders: Higher Education Authority (HEA)
    URI:

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