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    Generation of homoplasmic plastid transformants of a commercial cultivar of potato (Solanum tuberosum L.)


    Nguyen, Thanh Thi and Nugent, Gregory and Cardi, Teodoro and Dix, Philip (2005) Generation of homoplasmic plastid transformants of a commercial cultivar of potato (Solanum tuberosum L.). Plant Science, 168. pp. 1495-1450. ISSN 0168-9452

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    Abstract

    This report describes the integration and expression of foreign genes into the plastid genome of a commercial cultivar of potato. Plastid transformation of potato was achieved using two tobacco specific plastid transformation vectors, pZS197 (Prrn/aadA/psbA3′) and pMSK18 (trc/gfp/Prrn/aadA/psbA3′). Selection was for spectinomycin resistance after biolistic delivery of plasmid DNA into leaf cells of Solanum tuberosum cv. Desiree. Ten transplastomic lines were obtained from 179 bombarded samples with vector pZS197 and four transplastomic lines selected out of 103 bombarded samples with vector pMSK18. Southern blot and PCR analyses confirmed homoplasmy in the primary regenerants, and incorporation of the aadA and gfp genes into the potato plastid genome by two homologous recombination events via the flanking plastid DNA sequences. Fluorometric measurements confirmed GFP expression in leaves and tubers of pMSK18 lines. No transformants were obtained with a third tobacco vector, pNtcZ7 (Prrn/gfp/psbA3′/trc/aadA/rrnB-ter) in which the selectable marker gene is driven by a bacterial (trc) promoter, which does permit selection of plastid transformants in tobacco, and allows low level expression of the reporter gene, gfp, in potato.

    Item Type: Article
    Keywords: Plastid transformation; Chimeric aadA gene; gfp gene; Particle bombardment; Transplastomic potato;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 7365
    Identification Number: https://doi.org/10.1016/j.plantsci.2005.01.023
    Depositing User: Prof. Philip J. Dix
    Date Deposited: 23 Aug 2016 10:57
    Journal or Publication Title: Plant Science
    Publisher: Elsevier
    Refereed: Yes
    Funders: Higher Education Authority (HEA), European Union (EU) 5th Framework programme
    URI:

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