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    Plant-based strategies aimed at expressing HIV antigens and neutralizing antibodies at high levels. Nef as a case study


    Marusic, Carla and Vitale, Allessandro and Pedrazzini, Emanuela and Donini, Marcello and Frigerio, Lorenzo and Bock, Ralph and Dix, Philip and McCabe, Matthew S. and Bellucci, Michele and Benvenuto, Eugenio (2009) Plant-based strategies aimed at expressing HIV antigens and neutralizing antibodies at high levels. Nef as a case study. Transgenic Research, 18 (4). pp. 499-512. ISSN 0962-8819

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    Abstract

    The first evidence that plants represent a valid, safe and cost-effective alternative to traditional expression systems for large-scale production of antigens and antibodies was described more than 10 years ago. Since then, considerable improvements have been made to increase the yield of plant-produced proteins. These include the use of signal sequences to target proteins to different cellular compartments, plastid transformation to achieve high transgene dosage, codon usage optimization to boost gene expression, and protein fusions to improve recombinant protein stability and accumulation. Thus, several HIV/SIV antigens and neutralizing anti-HIV antibodies have recently been successfully expressed in plants by stable nuclear or plastid transformation, and by transient expression systems based on plant virus vectors or Agrobacterium-mediated infection. The current article gives an overview of plant expressed HIV antigens and antibodies and provides an account of the use of different strategies aimed at increasing the expression of the accessory multifunctional HIV-1 Nef protein in transgenic plants.

    Item Type: Article
    Keywords: HIV; Nef; Multi-component vaccine; Plant made pharmaceuticals; Recombinant proteins; Heterologous expression systems;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 7375
    Identification Number: https://doi.org/10.1007/s11248-009-9244-5
    Depositing User: Prof. Philip J. Dix
    Date Deposited: 24 Aug 2016 15:37
    Journal or Publication Title: Transgenic Research
    Publisher: Springer
    Refereed: Yes
    URI:

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