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    'Regeneration from leaf explants and protoplasts of Brassica oleracea var. botrytis (cauliflower)


    Chikkala, Veera R.N. and Nugent, Gregory D. and Dix, Philip and Stevenson, Trevor W. (2009) 'Regeneration from leaf explants and protoplasts of Brassica oleracea var. botrytis (cauliflower). Scientia Horticulturae, 119. pp. 330-334. ISSN 0304-4238

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    Abstract

    Adventitious shoot regeneration and protoplast isolation and culture were examined from leaf explants of in vitro shoot cultures of several cauliflower (Brassica oleracea var. botrytis) cultivars, sourced from Europe and Australia, was investigated with the aim to develop improved nuclear and plastid transformation protocols for this vegetable crop. Eight out of 10 cultivars regenerated shoots from at least 79% of leaf explants. Mesophyll protoplasts from leaves gave high yields and division frequencies. Growth of shoot cultures in large glass vessels with vented lids was the key factor in obtaining high protoplast division frequencies of up to 71% and at least 70% of protoplast calluses regenerating shoots.

    Item Type: Article
    Keywords: Cauliflower; Shoot regeneration; Protoplast culture;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 7380
    Identification Number: https://doi.org/10.1016/j.scienta.2008.07.036
    Depositing User: Prof. Philip J. Dix
    Date Deposited: 25 Aug 2016 15:54
    Journal or Publication Title: Scientia Horticulturae
    Publisher: Elsevier
    Refereed: Yes
    URI:

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