Donoghue, Pamela and Staunton, Lisa and Mullen, Edel and Manning, Gwen and Ohlendieck, Kay (2010) DIGE analysis of rat skeletal muscle proteins using nonionic detergent phase extraction of young adult versus aged gastrocnemius tissue. Journal of Proteomics, 73 (8). pp. 1441-1453. ISSN 1874-3919

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Abstract

Contractile weakness and loss of muscle mass are critical features of the aging process in mammalians. Age-related fibre wasting has a profound effect on muscle metabolism, fibre type distribution and the overall physiological integrity of the neuromuscular system. This study has used mass spectrometry-based proteomics to investigate the fate of the aging rat muscle proteome. Using nonionic detergent phase extraction, this report shows that the aged gastrocnemius muscle exhibits a generally perturbed protein expression pattern in both the detergent-extracted fraction and the aqueous protein complement from senescent muscle tissue. In the detergent-extracted fraction, the expression of ATP synthase, isocitrate dehydrogenase, enolase, tropomyosin and beta-actin was increased. Different isoforms of creatine kinase and prohibitin showed differential changes. In the aqueous fraction, malate dehydrogenase, sulfotransferase, triosephosphate isomerase, aldolase, cofilin-2 and lactate dehydrogenase showed increased levels. Interestingly, differential effects on dissimilar 2-D spots of the same protein species were shown for Cu/Zn superoxide dismutase, albumin, annexin A4 and phosphoglycolate phosphatase. Mitochondrial Hsp60, Hsp71 and nucleoside diphosphate kinase B exhibited a reduced abundance in aged muscle. The majority of altered proteins were found to be involved in mitochondrial metabolism, glycolysis, metabolic transportation, regulatory processes, the cellular stress response, detoxification mechanisms and muscle contraction.

Item Type:	Article
Keywords:	Aging; Detergent phase extraction; DIGE; Muscle proteomics; Sarcopenia;
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	7491
Identification Number:	https://doi.org/10.1016/j.jprot.2010.01.014
Depositing User:	Prof. Kay Ohlendieck
Date Deposited:	07 Oct 2016 10:42
Journal or Publication Title:	Journal of Proteomics
Publisher:	Elsevier
Refereed:	Yes
Funders:	Science Foundation Ireland (SFI), Health Research Board (HRB), Higher Education Authority (HEA)
URI:	http://www.sciencedirect.com
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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