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    Proteomic DIGE analysis of the mitochondria-enriched fraction from aged rat skeletal muscle


    O'Connell, Kathleen and Ohlendieck, Kay (2009) Proteomic DIGE analysis of the mitochondria-enriched fraction from aged rat skeletal muscle. Proteomics, 9 (24). pp. 5509-5524. ISSN 1615-9853

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    Abstract

    Skeletal muscle aging is associated with a loss in tissue mass and contractile strength, as well as fiber type shifting and bioenergetic adaptation processes. Since mitochondria represent the primary site for energy generation via oxidative phosphorylation, we investigated potential changes in the expression pattern of the mitochondrial proteome using the highly sensitive DIGE approach. The comparative analysis of the mitochondria-enriched fraction from young adult versus aged muscle revealed an age-related change in abundance for 39 protein species. MS technology identified the majority of altered proteins as constituents of muscle mitochondria. An age-dependent increase was observed for NADH dehydrogenase, the mitochondrial inner membrane protein mitofilin, peroxiredoxin isoform PRX-III, ATPase synthase, succinate dehydrogenase, mitochondrial fission protein Fis1, succinate-coenzyme A ligase, acyl-coenzyme A dehydrogenase, porin isoform VDAC2, ubiquinol-cytochrome c reductase core I protein and prohibitin. Immunoblotting, enzyme testing and confocal microscopy were used to validate proteomic findings. The DIGE-identified increase in key mitochondrial elements during aging agrees with the concept that sarcopenia is associated with a shift to a slower contractile phenotype and more pronounced aerobic-oxidative metabolism. This suggests that mitochondrial markers are reliable candidates that should be included in the future establishment of a biomarker signature of skeletal muscle aging.

    Item Type: Article
    Keywords: Aging; Animal proteomics; DIGE; Mitochondria; Sarcopenia; Skeletal muscle proteomics;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 7496
    Identification Number: 0.1002/pmic.200900472
    Depositing User: Prof. Kay Ohlendieck
    Date Deposited: 07 Oct 2016 10:41
    Journal or Publication Title: Proteomics
    Publisher: Wiley
    Refereed: Yes
    Funders: Science Foundation Ireland (SFI), Health Research Board (HRB), Higher Education Authority (HEA)
    URI:

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