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    Development of an implantable d-serine biosensor for in vivo monitoring using mammalian d-amino acid oxidase on a poly (o-phenylenediamine) and Nafion-modified platinum–iridium disk electrode


    Zaina, Zainiharyati M. and O'Neill, Robert D. and Lowry, John P. and Pierce, Kenneth W. and Tricklebank, Mark and Dewae, Aidiahmad and Ab Ghani, Sulaiman (2010) Development of an implantable d-serine biosensor for in vivo monitoring using mammalian d-amino acid oxidase on a poly (o-phenylenediamine) and Nafion-modified platinum–iridium disk electrode. Biosensors and Bioelectronics, 25 (6). pp. 1454-1459. ISSN 0956-5663

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    Abstract

    d-serine has been implicated as a brain messenger, promoting not only neuronal signalling but also synaptic plasticity. Thus, a sensitive tool for d-serine monitoring in brain is required to understand the mechanisms of d-serine release from glia cells. A biosensor for direct fixed potential amperometric monitoring of d-serine incorporating mammalian d-amino acid oxidase (DAAO) immobilized on a Nafion coated poly-ortho-phenylenediamine (PPD) modified Pt–Ir disk electrode was therefore developed. The combined layers of PPD and Nafion enhanced the enzyme activity and biosensor efficiency by ∼2-fold compared with each individual layer. A steady state response time (t90%) of 0.7 ± 0.1 s (n = 8) and limit of detection 20 ± 1 nM (n = 8) were obtained. Cylindrical geometry showed lower sensitivity compared to disk geometry (61 ± 7 μA cm−2 mM−1, (n = 4), R2 = 0.999). Interference by ascorbic acid (AA), the main interference species in the central nervous system and other neurochemical electroactive molecules was negligible. Implantation of the electrode and microinjection of d-serine into rat brain striatal extracellular fluid demonstrated that the electrode was capable of detecting d-serine in brain tissue in vivo.

    Item Type: Article
    Keywords: Microbiosensor; d-serine; d-amino acid oxidase; Implantable; Neurochemical monitoring;
    Academic Unit: Faculty of Science and Engineering > Chemistry
    Item ID: 8027
    Identification Number: https://doi.org/10.1016/j.bios.2009.10.049
    Depositing User: John Lowry
    Date Deposited: 13 Mar 2017 16:59
    Journal or Publication Title: Biosensors and Bioelectronics
    Publisher: Elsevier
    Refereed: Yes
    Funders: Science Foundation Ireland (SFI), Eli Lilly
    URI:

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