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    Development and characterization in vitro of a catalase-based biosensor for hydrogen peroxide monitoring


    O'Brien, Kylie B. and Killoran, Sarah J. and O'Neill, Robert D. and Lowry, John P. (2007) Development and characterization in vitro of a catalase-based biosensor for hydrogen peroxide monitoring. Biosensors and Bioelectronics, 22 (12). pp. 2994-3000. ISSN 0956-5663

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    Abstract

    There is increasing evidence that hydrogen peroxide (H2O2) may act as a neuromodulator in the brain, as well as contributing to neurodegeneration in diseased states, such as Parkinson's disease. The ability to monitor changes in endogenous H2O2in vivo with high temporal resolution is essential in order to further elucidate the roles of H2O2 in the central nervous system. Here, we describe the in vitro characterization of an implantable catalase-based H2O2 biosensor. The biosensor comprises two amperometric electrodes, one with catalase immobilized on the surface and one without enzyme (blank). The analytical signal is then the difference between the two electrodes. The H2O2 sensitivity of various designs was compared, and ranged from 0 to 56 ± 4 mA cm−2 M−1. The most successful design incorporated a Nafion® layer followed by a poly-o-phenylenediamine (PPD) polymer layer. Catalase was adsorbed onto the PPD layer and then cross-linked with glutaraldehyde. The ability of the biosensors to exclude interference from ascorbic acid, and other interference species found in vivo, was also tested. A variety of the catalase-based biosensor designs described here show promise for invivo monitoring of endogenous H2O2 in the brain.

    Item Type: Article
    Keywords: Catalase; Hydrogen peroxide; Brain; Nafion®; Poly(o-phenylenediamine); Reactive oxygen species (ROS);
    Academic Unit: Faculty of Science and Engineering > Chemistry
    Item ID: 8036
    Identification Number: https://doi.org/10.1016/j.bios.2006.12.020
    Depositing User: John Lowry
    Date Deposited: 21 Mar 2017 16:20
    Journal or Publication Title: Biosensors and Bioelectronics
    Publisher: Elsevier
    Refereed: Yes
    Funders: Health Research Board (HRB), Science Foundation Ireland (SFI)
    URI:

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