Byrne, Jonathan and Velasco-Torrijos, Trinidad and Reinhardt, Robert (2014) Development and validation of a novel stability-indicating HPLC method for the simultaneous assay of betamethasone-17-valerate, fusidic acid, potassium sorbate, methylparaben and propylparaben in a topical cream preparation. Journal of Pharmaceutical and Biomedical Analysis, 96. pp. 111-117. ISSN 1873-264X

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Abstract

A novel stability-indicating reversed phase high performance liquid chromatographic (RP-HPLC) method for the simultaneous assay of betamethasone-17-valerate, fusidic acid and potassium sorbate as well as methyl- and propylparaben in a topical cream preparation has been developed. A 100 mm × 3.0 mm ID. Ascentis Express C18 column maintained at 30 °C and UV detection at 240 nm were used. A gradient programme was employed at a flow-rate of 0.75 ml/min. Mobile phase A comprised of an 83:17 (v/v) mixture of acetonitrile and methanol and mobile phase B of a 10 g/l solution of 85% phosphoric acid in purified water. The method has been validated according to current International Conference on Harmonisation (ICH) guidelines and applied during formulation development and stability studies. The procedure has been shown to be stability-indicating for the topical cream.

Item Type:	Article
Keywords:	HPLC; Fusidic acid; Betamethasone-17-valerate; Preservatives; Development; Validation;
Academic Unit:	Faculty of Science and Engineering > Chemistry
Item ID:	10741
Identification Number:	https://doi.org/10.1016/j.jpba.2014.03.005
Depositing User:	Dr. Trinidad Velasco-Torrijos
Date Deposited:	23 Apr 2019 14:29
Journal or Publication Title:	Journal of Pharmaceutical and Biomedical Analysis
Publisher:	Elsevier
Refereed:	Yes
URI:	https://www.sciencedirect.com
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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