O'Flaherty, Roisin and Harbison, Aoife M. and Hanley, Philip J. and Taron, Christopher H. and Fadda, Elisa and Rudd, Pauline M. (2017) Aminoquinoline Fluorescent Labels Obstruct Efficient Removal ofN‑Glycan Core α(1−6) Fucose by Bovine Kidney α‑L‑Fucosidase (BKF). Journal of Proteome Research, 16. pp. 4237-4243. ISSN 1535-3893

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Abstract

Here we report evidence that new aminoquinoline N-glycan fluorescent labels interfere with the release of core α(1−6) fucose from N-glycans by bovine kidney α-L-fucosidase (BKF). BKF is a commonly employed exoglycosidase for core α(1−6) fucose determination. Molecular simulations of the bound and unbound Fuc-α(1−6)-GlcNAc, where GlcNAc is situated at the reducing end for all N-glycans, suggest that the reduced BKF activity may be due to a nonoptimal fit of the highest populated conformers in the BKF active binding site at room temperature.Population analysis and free energy estimates suggest that an enhanced flexibility of the labeled sugar, which facilitates recognition and binding, can be achievable with extended reaction conditions. We provide these experimental conditions using a sequential exoglycosidase digestion array using high concentrations of BKF.

Item Type:	Article
Keywords:	aminoquinoline (AQC;) bovine kidney fucosidase (BKF); N-glycan ultra performance; liquid chromatography (UPLC);
Academic Unit:	Faculty of Science and Engineering > Chemistry
Item ID:	11518
Identification Number:	https://doi.org/10.1021/acs.jproteome.7b00580
Depositing User:	Elisa Fadda
Date Deposited:	30 Oct 2019 13:58
Journal or Publication Title:	Journal of Proteome Research
Publisher:	American Chemical Society
Refereed:	Yes
URI:	Publisher
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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