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    2-Nitroimidazole based fluorescent probes for nitroreductase; monitoring reductive stress in cellulo


    Ao, Xiang and Bright, Sandra A. and Taylor, N.C. and Elmes, Robert B.P. (2017) 2-Nitroimidazole based fluorescent probes for nitroreductase; monitoring reductive stress in cellulo. Organic & Biomolecular Chemistry, 15. pp. 6104-6108. ISSN 1477-0520

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    Abstract

    Two 2-nitroimidazole-1,8-naphthalimide conjugates, 1 and 2, have been synthesised as fluorescence probes for the detection of reductive stress in HeLa cells. The 4-substituted derivative 1 was shown to act as a highly sensitive and selective substrate for nitroreductase where it exhibited a clear blue to green ratiometric fluorescence response visible to the naked eye. Moreover, biological studies demonstrated 1 could be activated in cellulo where the impact of reductive stress was easily monitored using confocal microscopy and flow cytommetry.

    Item Type: Article
    Additional Information: Cite as: Org. Biomol. Chem., 2017, 15, 6104
    Keywords: 2-Nitroimidazole; fluorescent probes; nitroreductase; monitoring reductive stress; HeLa cells;
    Academic Unit: Faculty of Science and Engineering > Chemistry
    Item ID: 11631
    Identification Number: https://doi.org/10.1039/C7OB01406F
    Depositing User: Robert Elmes
    Date Deposited: 05 Nov 2019 15:25
    Journal or Publication Title: Organic & Biomolecular Chemistry
    Publisher: Royal Society of Chemistry
    Refereed: Yes
    URI:

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