Fuku, Xolile and Singh, Baljit and Ajayi, Rachel F. and Jijana, Abongile N. and Baker, Priscilla and Dempsey, Eithne and Iwuoha, Emmanuel (2015) A gallium telluride quantum dots bioelectrode system for human epidermal growth factor receptor-2 (Her2/neu) oncogene signalling. Analytical Methods, 7 (15). pp. 6114-6124. ISSN 1759-9660

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Abstract

Human epidermal growth factor receptor 2 (Her2/neu) is a biomarker that is overexpressed in human breast cancers. A quantum dots (QDs) -based genosensor for Her2/neu oncogene was developed with gallium telluride QDs and amine-terminated probe ssDNA (NH2-5′-AATTCCAGTGGCCATCAA-3′), that is complementary to the DNA sequence of a section of the ERF gene of HER-2/neu (i.e. 5′-GAACATGAAGGACCGGTGGGC-3′). The QDs were highly crystalline, 6 nm (from XRD) in size and had band gap values of 3.3 eV and 3.5 eV calculated from fluorescence emission and UV-visible absorption data, respectively. The sensor sensitivity and limit of detection values were 10.0 μA ng−1 mL−1 and 0.2 pg mL−1 Her2/neu oncogene, respectively.

Item Type:	Article
Keywords:	Breast Cancer; Immunohistochemistry; Fabrication; Metastasis; Expression; Biomarkers; Pathology; Electrode; Toxicity; Invasion;
Academic Unit:	Faculty of Science and Engineering > Chemistry
Item ID:	12523
Identification Number:	https://doi.org/10.1039/c5ay00962f
Depositing User:	Eithne Dempsey
Date Deposited:	05 Mar 2020 16:51
Journal or Publication Title:	Analytical Methods
Publisher:	Royal Society of Chemistry
Refereed:	Yes
URI:	Publisher
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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