Sedgwick, Adam C. and Hayden, Alex and Hill, Barry and Bull, Steven D. and Elmes, Robert B.P. and James, Tony D. (2018) A simple umbelliferone based fluorescent probe for the detection of nitroreductase. Frontiers of Chemical Science and Engineering, 12. pp. 311-314. ISSN 2095-0187

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Abstract

A simple nitrobenzyl-umbelliferone (NCOU1) was synthesised containing a nitroreductase (NTR) trigger moiety. The presence of NTR, resulted in the fragmentation of the parent molecule and release of the highly emissive fluorophore umbelliferone via an NTR-catalyzed reduction of the nitro group. In the presence of the NTR enzyme, NCOU1 gave rise to a 5-fold increase in fluorescence intensity at 455 nm and was selective for NTR over other reductive enzymes. These results indicate that NCOU1 can be used as a simple assay for the detection of NTR.

Item Type:	Article
Keywords:	Off-On Probe; Hytixic Tumor; Cancer Therapy; Living Cells; Peroxynitrite;
Academic Unit:	Faculty of Science and Engineering > Chemistry
Item ID:	12530
Identification Number:	https://doi.org/10.1007/s11705-017-1697-0
Depositing User:	Robert Elmes
Date Deposited:	06 Mar 2020 16:52
Journal or Publication Title:	Frontiers of Chemical Science and Engineering
Publisher:	Springer
Refereed:	Yes
URI:	Publisher
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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