Tang, Zhengyuan and Barton, Sinead and Ward, Tomas E. and Lowry, John P. and Doran, Michelle M. and Byrne, Hugh J. and Hennelly, Bryan M. (2018) Multicomponent analysis using a confocal Raman microscope. Applied Optics, 57 (22). ISSN 1559-128X
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Abstract
Measuring the concentration of multiple chemical components in a low-volume aqueous mixture by Raman spectroscopy has received significant interest in the literature. All of the contributions to date focus on the design of optical systems that facilitate the recording of spectra with high signal-to-noise ratio by collecting as many Raman scattered photons as possible. In this study, the confocal Raman microscope setup is investigated for multicomponent analysis. Partial least-squares regression is used to quantify physiologically relevant aqueous mixtures of glucose, lactic acid, and urea. The predicted error is 17.81 mg/dL for glucose, 10.6 mg/dL for lactic acid, and 7.6 mg/dL for urea, although this can be improved with increased acquisition times. A theoretical analysis of the method is proposed, which relates the numerical aperture and the magnification of the microscope objective, as well as the confocal pinhole size, to the performance of the technique.
| Item Type: | Article |
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| Keywords: | confocal microscopy; Raman spectroscopy |
| Academic Unit: | Faculty of Science and Engineering > Computer Science Faculty of Science and Engineering > Chemistry Faculty of Science and Engineering > Electronic Engineering |
| Item ID: | 13131 |
| Identification Number: | https://doi.org/10.1364/AO.57.00E118 |
| Depositing User: | Dr. Bryan Hennelly |
| Date Deposited: | 22 Jul 2020 14:11 |
| Journal or Publication Title: | Applied Optics |
| Publisher: | Optical Society of America |
| Refereed: | Yes |
| Funders: | Science Foundation Ireland (SFI) |
| URI: | |
| Use Licence: | This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here |
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