Kelch, Jessica and Delaney, Aoife and Kelleher, Fintan and Baker, Priscilla and Iwuoha, Emmanuel and Dempsey, Eithne
(2020)
Impedimetric and electrochemical evaluation of a new redox active steroid derivative for hormone immunosensing.
Biosensors and Bioelectronics, 150 (111876).
ISSN 0956-5663
Abstract
Preparation and electrochemical interrogation of a novel redox active progesterone derivative progesterone
thiosemicarbazone (PATC) is presented here together with an investigation into its suitability as conjugate in
progesterone hormone immunosensing. PATC synthesis involved a condensation reaction between progesterone
acetate and thiosemicarbazone hydrochloride. Voltammetric and pulse techniques confirmed the redox behaviour of the new compound with concentration and scan rate dependant irreversible behaviour evident at glassy
carbon and gold transducers - ko (standard heterogeneous rate constant) was 2.56 � 10-3 cm2
/s (ν ¼ 100 mV/s in
non-aqeuous media). Bioaffinity studies towards anti-progesterone antibodies involved a competitive ELISA
format (optical) which confirmed recognition of the new progesterone derivative. Electrochemical impedance
spectroscopy was employed as an interrogation technique in order to establish optimum binding and surface
conditions for progesterone antigen-antibody interaction with the assistance of a redox probe (potassium
hexacyanoferrate).
| Item Type: |
Article
|
| Additional Information: |
Funding: The authors would like to acknowledge funding under the Science
Foundation Ireland Investigator Programme 12/IP/1275, and mobility
funding under Marie Curie Actions – International Research Staff Exchange GA318053. Cite as: Jessica Kelch, Aoife Delaney, Fintan Kelleher, Priscilla Baker, Emmanuel Iwuoha, Eithne Dempsey, Impedimetric and electrochemical evaluation of a new redox active steroid derivative for hormone immunosensing,
Biosensors and Bioelectronics, Volume 150, 2020, 111876, ISSN 0956-5663, https://doi.org/10.1016/j.bios.2019.111876. |
| Keywords: |
Electrochemical; impedance; spectroscopy;
Progesterone; thiosemicarbazone;
Progesterone; immunoassay; |
| Academic Unit: |
Faculty of Science and Engineering > Research Institutes > Human Health Institute |
| Item ID: |
13975 |
| Identification Number: |
https://doi.org/10.1016/j.bios.2019.111876 |
| Depositing User: |
Eithne Dempsey
|
| Date Deposited: |
08 Feb 2021 17:19 |
| Journal or Publication Title: |
Biosensors and Bioelectronics |
| Publisher: |
Elsevier |
| Refereed: |
Yes |
| Funders: |
Science Foundation Ireland Investigator Programme |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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