Warren, Susan and Munteanu, Grigore and Rathod, Dhanraj and McCormac, Timothy and Dempsey, Eithne
(2019)
Scanning electrochemical microscopy imaging of poly (3,4-ethylendioxythiophene)/thionine electrodes for lactate detection via NADH electrocatalysis.
Biosensors and Bioelectronics, 137.
pp. 15-24.
ISSN 0956-5663
Abstract
Herein we report the use of scanning electrochemical microscopy (SECM) together with electrochemical and
spectroscopic techniques to develop and characterise a stable and uniformly reactive chemically modified platinum electrode for NADH electrocatalysis. In order to achieve this, a range of different approaches for thionine
entrapment within an electropolymerised poly (3,4-ethylendioxythiophene) (PEDOT) film were evaluated using
SECM imaging in the presence of NADH, demonstrating the uniformity of the reactive layer towards NADH
oxidation. The effect of electrolyte type and time scale employed during PEDOT electropolymerisation was
examined with respect to thionine loading and the resulting charge transport diffusion coefficient (DCT) estimated via chronoamperometry. These studies indicated a decrease in DCT as thionine loading increased within
the PEDOT film, suggesting that charge transport was diffusion limited within the film. Additionally, thionine
functionalised nanotubes were formed, providing a stable support for lactate dehydrogenase entrapment while
lowering the rate of thionine leaching, determined via SECM imaging. This enabled lactate determination at
Eapp = 0.0 V vs Ag/AgCl over the range 0.25–5 mM in the presence of 1 mM NAD+.
| Item Type: |
Article
|
| Additional Information: |
Cite as: Susan Warren, Grigore Munteanu, Dhanraj Rathod, Timothy McCormac, Eithne Dempsey,
Scanning electrochemical microscopy imaging of poly (3,4-ethylendioxythiophene)/thionine electrodes for lactate detection via NADH electrocatalysis,
Biosensors and Bioelectronics,
Volume 137,
2019,
Pages 15-24,
ISSN 0956-5663,
https://doi.org/10.1016/j.bios.2019.04.042. |
| Keywords: |
Thionine;
Poly-3,4-ethylenedioxythiophene (PEDOT);
Multiwalled carbon nanotubes (MWNT);
Lactate biosensor;
Lactate dehydrogenase (LDH);
Scanning electrochemical microscopy (SECM);
Charge transport studies; |
| Academic Unit: |
Faculty of Science and Engineering > Chemistry |
| Item ID: |
13978 |
| Identification Number: |
https://doi.org/10.1016/j.bios.2019.04.042 |
| Depositing User: |
Eithne Dempsey
|
| Date Deposited: |
09 Feb 2021 16:44 |
| Journal or Publication Title: |
Biosensors and Bioelectronics |
| Publisher: |
Elsevier |
| Refereed: |
Yes |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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