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    The Influence of N-Linked Glycans on the Molecular Dynamics of the HIV-1 gp120 V3 Loop


    Wood, Natasha T. and Fadda, Elisa and Davis, Robert and Grant, Oliver C. and Martin, Joanne C. and Woods, Robert J. and Travers, Simon A. (2013) The Influence of N-Linked Glycans on the Molecular Dynamics of the HIV-1 gp120 V3 Loop. PLoS ONE, 8 (11). e80301. ISSN 1932-6203

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    Abstract

    N-linked glycans attached to specific amino acids of the gp120 envelope trimer of a HIV virion can modulate the binding affinity of gp120 to CD4, influence coreceptor tropism, and play an important role in neutralising antibody responses. Because of the challenges associated with crystallising fully glycosylated proteins, most structural investigations have focused on describing the features of a non-glycosylated HIV-1 gp120 protein. Here, we use a computational approach to determine the influence of N-linked glycans on the dynamics of the HIV-1 gp120 protein and, in particular, the V3 loop. We compare the conformational dynamics of a non-glycosylated gp120 structure to that of two glycosylated gp120 structures, one with a single, and a second with five, covalently linked high-mannose glycans. Our findings provide a clear illustration of the significant effect that N-linked glycosylation has on the temporal and spatial properties of the underlying protein structure. We find that glycans surrounding the V3 loop modulate its dynamics, conferring to the loop a marked propensity towards a more narrow conformation relative to its non-glycosylated counterpart. The conformational effect on the V3 loop provides further support for the suggestion that N-linked glycosylation plays a role in determining HIV-1 coreceptor tropism.

    Item Type: Article
    Keywords: N-Linked Glycans; Molecular Dynamics; HIV-1 gp120 V3 Loop;
    Academic Unit: Faculty of Science and Engineering > Chemistry
    Item ID: 4817
    Identification Number: https://doi.org/10.1371/journal.pone.0080301
    Depositing User: Elisa Fadda
    Date Deposited: 12 Mar 2014 11:07
    Journal or Publication Title: PLoS ONE
    Publisher: Public Library of Science
    Refereed: Yes
    Funders: Science Foundation of Ireland (08/IN.1/B2070), European Research Development Fund, National Institute of Health (GM094919 (EUREKA)
    URI:

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