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    Phase Behavior of DPPC in a DNA-Calcium-Zwitterionic Lipid Complex Studied by Small-Angle X-ray Scattering


    McManus, Jennifer J. and Rädler, Joachim O. and Dawson, Kenneth A. (2003) Phase Behavior of DPPC in a DNA-Calcium-Zwitterionic Lipid Complex Studied by Small-Angle X-ray Scattering. Langmuir, 19 (23). pp. 9630-9637. ISSN 0743-7463

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    Abstract

    In the presence of DNA and calcium, multilamellar liposomes of DPPC when in excess form complexes in which two distinct lamellar lipid phases coexist. The structure and phase behavior of both of these have been investigated using high-resolution small-angle X-ray scattering. The coexisting lipid phases exist within the same precipitated condensate with the phase behavior of the unbound lipid being affected by the complexed lipid phase. While the thermotropic phase transitions of the uncomplexed lipid from Lβ‘-Pβ‘-Lα occur as expected, the transitions of the bound lipid are substantially altered. This is manifested as an increase in the main transition temperature, Tm, of the bound lipid, which has been observed before (Tarahovsky, Y. S.; Khusainova, R. S.; Gorelov, A. V.; Nicolaeva, T. I.; Deev, A. A.; Dawson, K. A.; Ivanitsky, G. R. FEBS Lett. 1996, 390, 133. Kharakoz, D. P.; Khusainova, R. S.; Gorelov, A. V.; Dawson, K. A. FEBS Lett. 1999, 446, 27). However, we also observe an apparent disappearance of the ripple phase (Pβ‘) with increasing calcium concentration in the bound lipid phase. In addition we are in a position to comment on the mechanism of formation for complexes prepared in this way.

    Item Type: Article
    Keywords: Phase Behavior; DPPC; DNA-Calcium-Zwitterionic Lipid Complex; Small-Angle X-ray Scattering; DNA; Calcium;
    Academic Unit: Faculty of Science and Engineering > Chemistry
    Item ID: 7898
    Identification Number: https://doi.org/10.1021/la034878q
    Depositing User: Jennifer McManus
    Date Deposited: 10 Feb 2017 15:44
    Journal or Publication Title: Langmuir
    Publisher: American Chemical Society
    Refereed: Yes
    URI:

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