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    Oxygen tolerance of an implantable polymer/enzyme composite glutamate biosensor displaying polycation-enhanced substrate sensitivity


    McMahon, Colm P. and Rocchitta, Gaia and Kirwan, Sarah M. and Killoran, Sarah J. and Serra, Pier A. and Lowry, John P. and O'Neill, Robert D. (2007) Oxygen tolerance of an implantable polymer/enzyme composite glutamate biosensor displaying polycation-enhanced substrate sensitivity. Biosensors and Bioelectronics, 22 (7). pp. 1466-1473. ISSN 0956-5663

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    Abstract

    Biosensors were fabricated at neutral pH by sequentially depositing the polycation polyethyleneimine (PEI), the stereoselective enzyme l-glutamate oxidase (GluOx) and the permselective barrier poly-ortho-phenylenediamine (PPD) onto 125-μm diameter Pt wire electrodes (Pt/PEI/GluOx/PPD). These devices were calibrated amperometrically at 0.7 V versus SCE to determine the Michaelis–Menten parameters for enzyme substrate, l-glutamate (Glu) and co-substrate, dioxygen. The presence of PEI produced a 10-fold enhancement in the detection limit for Glu (∼20 nM) compared with the corresponding PEI-free configurations (Pt/GluOx/PPD), without undermining their fast response time (∼2 s). Most remarkable was the finding that, although some designs of PEI-containing biosensors showed a 10-fold increase in linear region sensitivity to Glu, their oxygen dependence remained low.

    Item Type: Article
    Keywords: Glutamate oxidase; Polyethyleneimine; PEI; Poly(o-phenylenediamine); PPD; Michaelis–Menten; Brain monitoring; Neurochemistry;
    Academic Unit: Faculty of Science and Engineering > Chemistry
    Item ID: 8037
    Identification Number: https://doi.org/10.1016/j.bios.2006.06.027
    Depositing User: John Lowry
    Date Deposited: 22 Mar 2017 16:54
    Journal or Publication Title: Biosensors and Bioelectronics
    Publisher: Elsevier
    Refereed: Yes
    Funders: Science Foundation Ireland (SFI)
    URI:

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