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    Calibration of NO sensors for In Vivo Voltammetry: Laboratory synthesis of NO and the use of UV-visible spectroscopy in determining stock concentrations


    Brown, Finbar O. and Finnerty, Niall J. and Bolger, Fiachra B. and Millar, Julian and Lowry, John P. (2005) Calibration of NO sensors for In Vivo Voltammetry: Laboratory synthesis of NO and the use of UV-visible spectroscopy in determining stock concentrations. Analytical and Bioanalytical Chemistry, 381 (4). pp. 964-971. ISSN 1618-2642

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    Abstract

    The increasing scientific interest in nitric oxide (NO) necessitates the development of novel and simple methods of synthesising NO on a laboratory scale. In this study we have refined and developed a method of NO synthesis, using the neutral Griess reagent, which is inexpensive, simple to perform, and provides a reliable method of generating NO gas for in-vivo sensor calibration. The concentration of the generated NO stock solution was determined using UV–visible spectroscopy to be 0.28±0.01 mmol L−1. The level of NO2− contaminant, also determined using spectroscopy, was found to be 0.67±0.21 mmol L−1. However, this is not sufficient to cause any considerable increase in oxidation current when the NO stock solution is used for electrochemical sensor calibration over physiologically relevant concentrations; the NO sensitivity of bare Pt-disk electrodes operating at +900 mV (vs. SCE) was 1.08 nA μmol−1 L, while that for NO2− was 5.9×10−3 nA μmol−1 L. The stability of the NO stock solution was also monitored for up to 2 h after synthesis and 30 min was found to be the time limit within which calibrations should be performed.

    Item Type: Article
    Keywords: Nitric oxide; Laboratory synthesis; UV–visible spectroscopy; In-vivo voltammetry; Electrochemical sensors; Calibrations;
    Academic Unit: Faculty of Science and Engineering > Chemistry
    Item ID: 8041
    Identification Number: https://doi.org/10.1007/s00216-004-2964-8
    Depositing User: John Lowry
    Date Deposited: 22 Mar 2017 16:47
    Journal or Publication Title: Analytical and Bioanalytical Chemistry
    Publisher: Springer Verlag
    Refereed: Yes
    Funders: Analytical Chemistry Trust Fund (Royal Society of Chemistry, RSC), Engineering and Physical Sciences Research Council (EPSRC) UK, Enterprise Ireland (EI), Health Research Board (HRB), NUI Maynooth
    URI:

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