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    Microelectrochemical Sensors for In Vivo Brain Analysis: An Investigation of Procedures for Modifying Pt Electrodes Using Nafion®


    Brown, Finbar O. and Lowry, John P. (2003) Microelectrochemical Sensors for In Vivo Brain Analysis: An Investigation of Procedures for Modifying Pt Electrodes Using Nafion®. Analyst, 128. pp. 700-705. ISSN 0003-2654

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    Abstract

    Various Nafion® coating procedures were examined in order to design a simple and reproducible coating method to maximise permselective characteristics, and thus eliminate signals from electroactive interferents, in sensors designed for direct in vivo measurements in the brain. Interferents investigated included ascorbic acid (AA), the principal endogenous electroactive interferent present in the brain, and uric acid. Application of the Nafion® (5% commercial solution) using a thermally annealing procedure involving 5 pre-coats, and 2 subsequent dip-bake layers resulted in elimination of interferent signals. It also produced complete blocking of the signal for the neurotransmitter dopamine. The optimum time and temperature for annealing was found to be 5 min at 210 °C. An examination of shelf life over two weeks indicated negligible AA interference over this period. Preliminary investigations with respect to the potential use of these Nafion®-modified Pt electrodes in the design of implantable, first generation, peroxide detecting biosensors indicated that the modified electrode had no effect on O2 permeability but did produce a significant decrease in H2O2 sensitivity. While this may preclude their use in biosensor development they may be more suitable for detection of gaseous neurochemicals such as nitric oxide.

    Item Type: Article
    Keywords: Microelectrochemical Sensors; In Vivo Brain Analysis; Modifying Pt Electrodes; Nafion®;
    Academic Unit: Faculty of Science and Engineering > Chemistry
    Item ID: 8046
    Identification Number: https://doi.org/10.1039/B300266G
    Depositing User: John Lowry
    Date Deposited: 22 Mar 2017 16:45
    Journal or Publication Title: Analyst
    Publisher: Royal Society of Chemistry
    Refereed: Yes
    Funders: Enterprise Ireland (EI), Health Research Board (HRB), NUI Maynooth
    URI:

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