MURAL - Maynooth University Research Archive Library



    Characterization in vitro and in vivo of the oxygen dependence of an enzyme/polymer biosensor for monitoring brain glucose


    Dixon, Blanaid M. and Lowry, John P. and O'Neill, Robert D. (2002) Characterization in vitro and in vivo of the oxygen dependence of an enzyme/polymer biosensor for monitoring brain glucose. Journal of Neuroscience Methods, 119 (2). pp. 135-142. ISSN 0165-0270

    [img]
    Preview
    Download (568kB) | Preview


    Share your research

    Twitter Facebook LinkedIn GooglePlus Email more...



    Add this article to your Mendeley library


    Abstract

    The oxygen dependence of a first generation amperometric biosensor was investigated in vitro and in vivo by monitoring its glucose response as a function of solution pO(2). The biosensor was a glucose oxidase (GOx) modified poly(o-phenylenediamine) coated Pt cylinder electrode (Pt/PPD/GOx) that has been designed for neurochemical analysis in vivo. Two types of oxygen probes were used: a self-calibrating commercial macroelectrode in vitro; and a carbon paste microelectrode in vivo. Calibrations in vitro showed that oxygen interference in the operation of Pt/PPD/GOx electrodes was minimal for concentrations of glucose (approximately 0.5 mM) and oxygen (approximately 50 microM) found in brain ECF. This observation was confirmed by simultaneous monitoring in vivo of brain glucose and oxygen in the awake rat. However, at levels of glucose normally found in peripheral tissues (approximately 5 mM), the oxygen dependence was severe. We conclude that the oxygen sensitivity of Pt/PPD/GOx biosensors does not preclude their reliable use in media containing low glucose levels, such as brain ECF.

    Item Type: Article
    Keywords: Poly(o-phenylenediamine); Enzyme modified electrode; Amperometry; Voltammetry in vivo; Glucose oxidase; Electropolymerization;
    Academic Unit: Faculty of Science and Engineering > Chemistry
    Item ID: 8047
    Identification Number: https://doi.org/10.1016/S0165-0270(02)00170-X
    Depositing User: John Lowry
    Date Deposited: 22 Mar 2017 16:45
    Journal or Publication Title: Journal of Neuroscience Methods
    Publisher: Elsevier
    Refereed: Yes
    Funders: Enterprise Ireland (EI), Health Research Board (HRB)
    URI:

    Repository Staff Only(login required)

    View Item Item control page

    Downloads

    Downloads per month over past year