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    Behaviourally induced changes in ectracellular levels of brain glutamate monitored at 1 s resolution with an implanted biosensor


    Lowry, John P. and Ryan, Michael R. and O'Neill, Robert D. (1998) Behaviourally induced changes in ectracellular levels of brain glutamate monitored at 1 s resolution with an implanted biosensor. Analytical Communications, 35. pp. 87-89. ISSN 1359-7337

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    Abstract

    A biosensor for L-glutamic acid (Glu), constructed by immobilisation of L-glutamate oxidase onto lipid-coated 60 µm radius Pt wire with poly(o-phenylenediamine), was used amperometrically in attempts to detect glutamate in brain extracellular fluid (ECF) in the awake, freely moving rat at 1 s intervals. An implanted carbon paste electrode served to monitor simultaneous changes in brain ascorbate (AA). There were spontaneous, parallel changes in both signals, depending on the behavioural state of the animal, giving rise to a significant positive correlation coefficient between the two variables. The application of a mild stressor (10 s tail pinch) caused an immediate increase in both signals. The effects of inhibiting energy metabolism, together with an existing hypothesis linking Glu and AA in the ECF, were used to conclude that AA does not interfere electrochemically with the biosensor in vivo, that there are parallel changes in extracellular Glu and AA concentrations under normal physiological conditions, and that sub-micromolar physiologically related changes in brain Glu could be detected in the ECF several days after implantation of the electrodes.

    Item Type: Article
    Keywords: Behaviourally induced changes; ectracellular levels; brain glutamate; 1 s resolution; implanted biosensor; L-glutamic acid (Glu);
    Academic Unit: Faculty of Science and Engineering > Chemistry
    Item ID: 8055
    Identification Number: https://doi.org/10.1039/A708747K
    Depositing User: John Lowry
    Date Deposited: 23 Mar 2017 16:52
    Journal or Publication Title: Analytical Communications
    Publisher: Royal Society of Chemistry
    Refereed: Yes
    URI:

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