MURAL - Maynooth University Research Archive Library



    Continuous monitoring of extracellular glucose concentrations in the striatum of freely moving rats with an implanted glucose biosensor


    Lowry, John P. and O'Neill, Robert D. and Boutelle, M.G. and Fillenz, Marianne (1998) Continuous monitoring of extracellular glucose concentrations in the striatum of freely moving rats with an implanted glucose biosensor. Journal of Neurochemistry, 70 (1). pp. 391-396. ISSN 0022-3042

    [img]
    Preview
    Download (572kB) | Preview


    Share your research

    Twitter Facebook LinkedIn GooglePlus Email more...



    Add this article to your Mendeley library


    Abstract

    We have used a glucose oxidase-based sensor implanted in the striatum of freely moving rats to determine the concentration of extracellular glucose in two distinct ways. With a modification of the zero net flux method, in which different concentrations of glucose are infused through a dialysis probe glued to the biosensor, we calculated the concentration at which there was no change in glucose current by regression analysis; this gave a concentration of 0.351 ± 0.016 mM. Calculating the concentration from the basal current and the in vitro calibration of the biosensor was not significantly different from this. The basal extracellular glucose concentration determined by either method remained constant over a period of several days. Infusion of 50 µM veratridine through the adjacent dialysis probe caused a steep decrease in glucose current as soon as the drug reached the brain in contrast to the delayed fall (7.5 min) seen with microdialysis in previous experiments from this laboratory. These results demonstrate that this biosensor provides a direct, real-time measure of the extracellular concentration of glucose.

    Item Type: Article
    Keywords: Glucose biosensor; Brain glucose concentration; Veratridine; Rat striatum;
    Academic Unit: Faculty of Science and Engineering > Chemistry
    Item ID: 8057
    Identification Number: https://doi.org/10.1046/j.1471-4159.1998.70010391.x
    Depositing User: John Lowry
    Date Deposited: 23 Mar 2017 16:52
    Journal or Publication Title: Journal of Neurochemistry
    Publisher: Blackwell Publishing Ltd.
    Refereed: Yes
    URI:

    Repository Staff Only(login required)

    View Item Item control page

    Downloads

    Downloads per month over past year