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    Plant Tissue Culture Manual - Use of Chemical and Physical Mutagens In vitro


    Dix, Philip (1993) Plant Tissue Culture Manual - Use of Chemical and Physical Mutagens In vitro. Manual. Kluwer Academic Publishers.

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    Abstract

    In the twenty years since the first unequivocal reports (including inheritance data) on the selection of mutants in vitro [ !, 2], a large number of mutants have been isolated, and reviews have frequently updated the progress, most extensively in a recent treatise [3]. The earliest reports made no use of deliberate mutagenesis, relying instead on the wide genetic variation exhibited by cultured cells, based on spontaneous mutation rates and possibly enhanced by genetic instabilities of rapidly dividing cells in culture [ 4]. The fact that the numbers worked in favour of selection of spontaneous mutants, when a dominant monogenic trait with a strong positive selection pressure was involved, probably delayed a critical evaluation of the role of chemical and physical mutagens in enhancing "mutation frequency". This was exacerbated by difficulties in reliable quantification of the latter term, given that single cell cultures were generally not in use, and colony forming potential (or plating efficiency) was usually profoundly influenced by viable cell density.

    Item Type: Monograph (Manual)
    Keywords: Plant Tissue Culture; Manual; Chemical and Physical Mutagens; In vitro;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 10057
    Depositing User: Prof. Philip J. Dix
    Date Deposited: 04 Oct 2018 14:03
    Publisher: Kluwer Academic Publishers
    URI:

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