Goslin, Kevin
(2019)
Characterization of the Ubiquitin
N-end Rule Pathway in Arabidopsis.
PhD thesis, National University of Ireland Maynooth.
Abstract
The control of intracellular protein homeostasis is essential for the ability of plants
to grow under different physiological conditions, as well as respond to various biotic
or abiotic stresses. One of the ways that cells achieve this equilibrium is through the
targeted proteolysis of proteins by the ubiquitin-proteasome system. A subset of
this system, termed the N-end rule pathway, relates the in vivo longevity of a
substrate protein to the nature of its N-terminal amino acid. Although the N-end rule
pathway is known to regulate numerous physiological processes in plants relatively
few substrates of the pathway have been identified to date. In this study
experiments were conducted aimed at identifying N-end rule substrates in the
model plant Arabidopsis thaliana. One group of candidate substrates is generated
after their proteolytic cleavage by a bacterial effector protein. The transient
expression of these candidate N-end rule substrates in tobacco coupled with
pathogen inoculation and biochemical methods led to the identification of a group of
protein fragments that are likely novel N-end rule substrates. Experiments were
also conducted towards developing a molecular tagging tool with the aim of
conducting a proteome-wide screen for N-end rule substrates. Additionally,
experiments were carried out to characterize a component of the Arabidopsis N-end
rule pathway by expressing this enzyme in the yeast S. cerevisiae under different
conditions. This study will allow for a further understanding of the involvement of
the N-end rule pathway in plant-pathogen interactions and provides several novel
N-end rule substrates for future experiments aimed at dissecting the diverse
functions of this pathway in plants.
| Item Type: |
Thesis
(PhD)
|
| Keywords: |
Ubiquitin N-end Rule Pathway; Arabidopsis; |
| Academic Unit: |
Faculty of Science and Engineering > Biology |
| Item ID: |
10860 |
| Depositing User: |
IR eTheses
|
| Date Deposited: |
10 Jun 2019 11:22 |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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