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    Increased chitin biosynthesis contributes to the resistance of Penicillium polonicum against the antifungal protein PgAFP


    Delgado, Josué and Owens, Rebecca A. and Doyle, Sean and Asensio, Miguel A. and Núñez, Felix (2016) Increased chitin biosynthesis contributes to the resistance of Penicillium polonicum against the antifungal protein PgAFP. Applied Microbiology and Biotechnology, 100 (1). pp. 371-383. ISSN 1432-0614

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    Abstract

    Antifungal proteins from molds have been proposed as a valuable tool against unwanted molds, but the resistance of some fungi limits their use. Resistance to antimicrobial peptides has been suggested to be due to lack of interaction with the mold or to a successful response. The antifungal protein PgAFP produced by Penicillium chrysogenum inhibits the growth of various ascomycetes, but not Penicillium polonicum. To study the basis for resistance to this antifungal protein, localization of PgAFP and metabolic, structural, and morphological changes were investigated in P. polonicum. PgAFP bound the outer layer of P. polonicum but not regenerated chitin, suggesting an interaction with specific molecules. Comparative two-dimensional gel electrophoresis (2D-PAGE) and comparative quantitative proteomics revealed changes in the relative abundance of several proteins from ribosome, spliceosome, metabolic, and biosynthesis of secondary metabolite pathways. The proteome changes and an altered permeability reveal an active reaction of P. polonicum to PgAFP. The successful response of the resistant mold seems to be based on the higher abundance of protein Rho GTPase Rho1 that would lead to the increased chitin deposition via cell wall integrity (CWI) signaling pathway. Thus, combined treatment with chitinases could provide a complementary means to combat resistance to antifungal proteins.

    Item Type: Article
    Additional Information: This is the postprint version of the published article, which is available at: Delgado, J., Owens, R.A., Doyle, S. et al. Appl Microbiol Biotechnol (2016) 100: 371. https://doi.org/10.1007/s00253-015-7020-4.
    Keywords: Antifungal proteins; Proteomics; Resistance; Penicillium polonicum; Chitin Cell wall integrity pathway;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 10912
    Identification Number: https://doi.org/10.1007/s00253-015-7020-4
    Depositing User: Rebecca Owens
    Date Deposited: 02 Jul 2019 15:16
    Journal or Publication Title: Applied Microbiology and Biotechnology
    Publisher: Springer
    Refereed: Yes
    URI:

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