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    Tracing field hybridization in Ryegrass species using microsatellite and morphological markers


    Ryan, Eimear M. and Mullins, Ewen and Burke, James and Downes, Martin and Meade, Conor (2006) Tracing field hybridization in Ryegrass species using microsatellite and morphological markers. Environmental Biosafety Research, 5 (2). pp. 111-117. ISSN 1635-7922

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    Abstract

    We have assessed the utility of morphological and microsatellite markers for tracing field hybridization between Lolium multiflorum and Lolium perenne in cereal-enclosed gene flow plots. The presence of awns on the inflorescence of F1 hybrids was found to be a reliable, but underscoring, indicator of L. multiflorum paternity in L. perenne derived seed as determined by inheritance of species-specific alleles at the microsatellite locus ‘H01 H06’ in these progeny. A positive correlation was evident in the experimental treatment between the number of pollen donor plants in a given plot and the frequency of hybrid F1 seed harvested from pollen receptor plants in that plot. These experiments have established the utility of naturally occurring heritable markers for the measurement of gene flow rates in field Ryegrass populations, with particular significance for risk assessment modeling of potential gene flow from transgenic grass cultivars.

    Item Type: Article
    Keywords: Lolium perenne; Lolium multiflorum; pollen flow; gene flow; molecular markers; risk assessment;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 10923
    Identification Number: https://doi.org/10.1051/ebr:2007001
    Depositing User: Dr. Conor Meade
    Date Deposited: 05 Jul 2019 16:00
    Journal or Publication Title: Environmental Biosafety Research
    Publisher: EDP Sciences
    Refereed: Yes
    URI:

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