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    Comprehensive Analysis of Aspergillus nidulans PKA Phosphorylome Identifies a Novel Mode of CreA Regulation


    Ribeiro, Liliane F.C. and Chelius, Cynthia and Boppidi, Karthik R. and Naik, Nisha S. and Hossain, Simin and Ramsay, Jessica J.J. and Kumar, Jyothi and Ribeiro, Lucas F. and Ostermeier, Marc and Tran, Bao and Goo, Young Ah and de Assis, Leandro José and Ulas, Mevlut and Bayram, Ozgur and Goldman, Gustavo H. and Lincoln, Stephen and Srivastava, Ranjan and Harris, Steven D. and Marten, Mark R. (2019) Comprehensive Analysis of Aspergillus nidulans PKA Phosphorylome Identifies a Novel Mode of CreA Regulation. mBio, 10 (2). e02825-18. ISSN 2150-7511

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    Abstract

    In filamentous fungi, an important kinase responsible for adaptation to changes in available nutrients is cyclic AMP (cAMP)-dependent protein kinase (protein kinase A [PKA]). This kinase has been well characterized at a molecular level, but its systemic action and direct/indirect targets are generally not well understood in filamentous fungi. In this work, we used a pkaA deletion strain (ΔpkaA) to identify Aspergillus nidulans proteins for which phosphorylation is dependent (either directly or indirectly) on PKA. A combination of phosphoproteomic and transcriptomic analyses revealed both direct and indirect targets of PKA and provided a global perspective on its function. One of these targets was the transcription factor CreA, the main repressor responsible for carbon catabolite repression (CCR). In the ΔpkaA strain, we identified a previously unreported phosphosite in CreA, S319, which (based on motif analysis) appears to be a direct target of Stk22 kinase (AN5728). Upon replacement of CreA S319 with an alanine (i.e., phosphonull mutant), the dynamics of CreA import to the nucleus are affected. Collectively, this work provides a global overview of PKA function while also providing novel insight regarding significance of a specific PKA-mediated phosphorylation event.

    Item Type: Article
    Additional Information: © 2019 Ribeiro et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ . Citation: Ribeiro LFC, Chelius C, Boppidi KR,Naik NS, Hossain S, Ramsey JJJ, Kumar J, RibeiroLF, Ostermeier M, Tran B, Ah Goo Y, de Assis LJ,Ulas M, Bayram O, Goldman GH, Lincoln S,Srivastava R, Harris SD, Marten MR. 2019.Comprehensive analysis ofAspergillus nidulansPKA phosphorylome identifies a novel mode ofCreA regulation. mBio 10:e02825-18.https://doi.org/10.1128/mBio.02825-18.
    Keywords: CreA; phosphoproteomic; phosphosite; signal transduction; transcriptomic;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 10995
    Identification Number: https://doi.org/10.1128/mBio.02825-18
    Depositing User: Ozgur Bayram
    Date Deposited: 28 Aug 2019 14:24
    Journal or Publication Title: mBio
    Publisher: American society for Microbiology
    Refereed: Yes
    URI:

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