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    Changes of global gene expression and secondary metabolite accumulation during light-dependent Aspergillus nidulans development


    Bayram, Ozgur and Fuessner, Kirstin and Dumkowa, Marc and Herrfurth, Cornelia and Feussner, Ivo and Braus, Gerhard H. (2016) Changes of global gene expression and secondary metabolite accumulation during light-dependent Aspergillus nidulans development. Fungal Genetics and Biology, 87. pp. 30-53. ISSN 1087-1845

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    Abstract

    Fungal development and secondary metabolite production are coordinated by regulatory complexes as the trimeric velvet complex. Light accelerates asexual but decreases sexual development of the filamentous fungus Aspergillus nidulans. Changes in gene expression and secondary metabolite accumulation in response to environmental stimuli have been the focus of many studies, but a comprehensive comparison during entire development is lacking. We compared snapshots of transcript and metabolite profiles during fungal development in dark or light. Overall 2.014 genes corresponding to 19% of the genome were differentially expressed when submerged vegetative hyphae were compared to surface development. Differentiation was preferentially asexual in light or preferentially sexual connected to delayed asexual development in dark. Light induces significantly gene expression within the first 24–48 h after the transfer to surfaces. Many light induced genes are also expressed in dark after a delay of up to two days, which might be required for preparation of enhanced sexual development. Darkness results in a massive transcriptional reprogramming causing a peak of lipid-derived fungal pheromone synthesis (psi factors) during early sexual development and the expression of genes for cell-wall degradation presumably to mobilize the energy for sexual differentiation. Accumulation of secondary metabolites like antitumoral terrequinone A or like emericellamide start under light conditions, whereas the mycotoxin sterigmatocystin or asperthecin and emodin appear under dark conditions during sexual development. Amino acid synthesis and pool rapidly drop after 72–96 h in dark. Subsequent initiation of apoptotic cell-death pathways in darkness happens significantly later than in light. This illustrates that fungal adaptation in differentiation and secondary metabolite production to light conditions requires the reprogramming of one fifth of the potential of its genome.

    Item Type: Article
    Keywords: Aspergillus nidulans; Development; Light regulation; Conidiation; Sexual development; Gene expression; Secondary metabolism;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 11004
    Identification Number: https://doi.org/10.1016/j.fgb.2016.01.004
    Depositing User: Ozgur Bayram
    Date Deposited: 02 Sep 2019 16:01
    Journal or Publication Title: Fungal Genetics and Biology
    Publisher: Elsevier Inc.
    Refereed: Yes
    URI:

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