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    The effect of entomopathogenic fungal culture filtrate on the immune response and haemolymph proteome of the large pine weevil, Hylobius abietis


    McNamara, Louise and Griffin, Christine and Fitzpatrick, David A. and Kavanagh, Kevin and Carolan, James C. (2018) The effect of entomopathogenic fungal culture filtrate on the immune response and haemolymph proteome of the large pine weevil, Hylobius abietis. Insect Biochemistry and Molecular Biology, 101. pp. 1-13. ISSN 0965-1748

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    Abstract

    The large pine weevil Hylobius abietis L. is a major forestry pest in 15 European countries, where it is a threat to 3.4 million hectares of forest. A cellular and proteomic analysis of the effect of culture filtrate of three entomopathogenic fungi (EPF) species on the immune system of H. abietis was performed. Injection with Metarhizium brunneum or Beauvaria bassiana culture filtrate facilitated a significantly increased yeast cell proliferation in larvae. Larvae co-injected with either Beauvaria caledonica or B. bassiana culture filtrate and Candida albicans showed significantly increased mortality. Together these results suggest that EPF culture filtrate has the potential to modulate the insect immune system allowing a subsequent pathogen to proliferate. Injection with EPF culture filtrate was shown to alter the abundance of protease inhibitors, detoxifing enzymes, antimicrobial peptides and proteins involved in reception/detection and development in H. abietis larvae. Larvae injected with B. caledonica culture filtrate displayed significant alterations in abundance of proteins involved in cellulolytic and other metabolic processes in their haemolymph proteome. Screening EPF for their ability to modulate the insect immune response represents a means of assessing EPF for use as biocontrol agents, particularly if the goal is to use them in combination with other control agents.

    Item Type: Article
    Additional Information: This is the postprint version of the published article, which is available at Namara, L.M., Griffin, C.T., Fitzpatrick, D., Kavanagh, K., Carolan, J.C., The effect of entomopathogenic fungal culture filtrate on the immune response and haemolymph proteome of the large pine weevil, Hylobius abietis, Insect Biochemistry and Molecular Biology (2018), doi: 10.1016/ j.ibmb.2018.07.001.
    Keywords: Large pine weevil; Entomopathogenic fungi; Proteomic; Transcriptome; Immunomodulation; Biocontrol;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 11018
    Identification Number: https://doi.org/10.1016/ j.ibmb.2018.07.001
    Depositing User: James Carolan
    Date Deposited: 05 Sep 2019 09:55
    Journal or Publication Title: Insect Biochemistry and Molecular Biology
    Publisher: Elsevier
    Refereed: Yes
    URI:

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