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    Life and death of proteins after protease cleavage: protein degradation by the N-end rule pathway


    Dissmeyer, Nico and Rivas, Susana and Graciet, Emmanuelle (2018) Life and death of proteins after protease cleavage: protein degradation by the N-end rule pathway. New Phytologist, 218 (3). pp. 929-935. ISSN 0028-646X

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    Abstract

    The activity and abundance of proteins within a cell are controlled precisely to ensure the regulation of cellular and physiological processes. In eukaryotes, this can be achieved by targeting specific proteins for degradation by the ubiquitinproteasome system. The N-end rule pathway, a subset of the ubiquitinproteasome system, targets proteins for degradation depending on the identity of a protein N-terminal residue or its post-translational modifications. Here, we discuss the most recent findings on the diversity of N-end rule pathways. We also focus on recently found defensive functions of the N-end rule pathway in plants. We then discuss the current understanding of N-end rule substrate formation by protease cleavage. Finally, we review state-of-the-art proteomics techniques used for N-end rule substrate identification, and discuss their usefulness and limitations for the discovery of the molecular mechanisms underlying the roles of the N-end rule pathway in plants.

    Item Type: Article
    Additional Information: © 2017 The Authors. Cite as: Dissmeyer, N. , Rivas, S. and Graciet, E. (2018), Life and death of proteins after protease cleavage: protein degradation by the N‐end rule pathway. New Phytol, 218: 929-935. doi:10.1111/nph.14619
    Keywords: arginylation; cysteine oxidation; N-degron; N-end rule pathway; N-recognins; N-terminomics; proteases; ubiquitin-proteasome system;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 11022
    Identification Number: https://doi.org/10.1111/nph.14619
    Depositing User: Emmanuelle Graciet
    Date Deposited: 05 Sep 2019 13:38
    Journal or Publication Title: New Phytologist
    Publisher: Wiley
    Refereed: No
    Funders: Agence Nationale de la Recherche, Department of Agriculture, Food and the Marine, Science Foundation Ireland (SFI), Virtual Irish Centre for Crop Improvement, European Regional Development Fund, Deutsche Forschungsgemeinschaft
    URI:

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