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    Gene network analysis of Arabidopsis thaliana flower development through dynamic gene perturbations


    Ó'Maoiléidigh, Diarmuid S. and Thomson, Bennett and Raganelli, Andrea and Wuest, Samuel E. and Ryan, Patrick T. and Kwasniewska, Kamila and Carles, Cristel C. and Graciet, Emmanuelle and Wellmer, Frank (2015) Gene network analysis of Arabidopsis thaliana flower development through dynamic gene perturbations. Plant Journal, 83. pp. 344-358. ISSN 1365-313X

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    Abstract

    Understanding how flowers develop from undifferentiated stem cells has occupied developmental biologists for decades. Key to unraveling this process is a detailed knowledge of the global regulatory hierarchies that control developmental transitions, cell differentiation and organ growth. These hierarchies may be deduced from gene perturbation experiments, which determine the effects on gene expression after specific disruption of a regulatory gene. Here, we tested experimental strategies for gene perturbation experiments during Arabidopsis thaliana flower development. We used artificial miRNAs (amiRNAs) to disrupt the functions of key floral regulators, and expressed them under the control of various inducible promoter systems that are widely used in the plant research community. To be able to perform genome‐wide experiments with stage‐specific resolution using the various inducible promoter systems for gene perturbation experiments, we also generated a series of floral induction systems that allow collection of hundreds of synchronized floral buds from a single plant. Based on our results, we propose strategies for performing dynamic gene perturbation experiments in flowers, and outline how they may be combined with versions of the floral induction system to dissect the gene regulatory network underlying flower development.

    Item Type: Article
    Additional Information: © 2015 The Authors. Cite as: Ó'Maoiléidigh, D. S., Thomson, B. , Raganelli, A. , Wuest, S. E., Ryan, P. T., Kwaśniewska, K. , Carles, C. C., Graciet, E. and Wellmer, F. (2015), Gene network analysis of Arabidopsis thaliana flower development through dynamic gene perturbations. Plant J, 83: 344-358. doi:10.1111/tpj.12878
    Keywords: flower development; Arabidopsis thaliana; gene regulatory network; gene perturbation; inducible promoter systems; artificial miRNAs; technical advance;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 11023
    Identification Number: https://doi.org/10.1111/tpj.12878
    Depositing User: Emmanuelle Graciet
    Date Deposited: 05 Sep 2019 13:50
    Journal or Publication Title: Plant Journal
    Publisher: Wiley
    Refereed: Yes
    Funders: Science Foundation Ireland (SFI), Centre National de la Recherche Scientifique, ChromFlow ANR JCJC, Irish Research Council (IRC)
    URI:

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