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    Next-Generation Sequencing Applied to Flower Development (ChIP-Seq)


    Graciet, Emmanuelle and Ó'Maoiléidigh, Diarmuid S. and Wellmer, Frank (2014) Next-Generation Sequencing Applied to Flower Development (ChIP-Seq). In: Flower Development. Methods in Molecular Biology (Methods and Protocols) (1110). Humana Press, pp. 413-429. ISBN 9781461494089

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    Abstract

    Over the past 20 years, classic genetic approaches have shown that the developmental program underlying flower formation involves a large number of transcriptional regulators. However, the target genes of these transcription factors, as well as the gene regulatory networks they control, remain largely unknown. Chromatin immunoprecipitation coupled to next-generation sequencing (ChIP-Seq), which allows the identification of transcription factor binding sites on a genome-wide scale, has been successfully applied to a number of transcription factors in Arabidopsis. The ChIP-Seq procedure involves chemical cross-linking of proteins to DNA, followed by chromatin fragmentation and immunoprecipitation of specific protein–DNA complexes. The regions of the genome bound by a specific transcription factor can then be identified after next-generation sequencing.

    Item Type: Book Section
    Additional Information: This is the preprint version of the published article, which is available at: Graciet E., Ó’Maoiléidigh D.S., Wellmer F. (2014) Next-Generation Sequencing Applied to Flower Development: ChIP-Seq. In: Riechmann J., Wellmer F. (eds) Flower Development. Methods in Molecular Biology (Methods and Protocols), vol 1110. Humana Press, New York, NY
    Keywords: Arabidopsis; flower development; ChIP-Seq; transcription factors;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 11024
    Identification Number: https://doi.org/10.1007/978-1-4614-9408-9_24
    Depositing User: Emmanuelle Graciet
    Date Deposited: 05 Sep 2019 14:52
    Journal or Publication Title: Flower Development
    Publisher: Humana Press
    Refereed: Yes
    URI:

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