Waldron, Rose and McGowan, Jamie and Gordon, Natasha and McCarthy, Charley G.P. and Mitchell, E. Bruce and Fitzpatrick, David A. (2019) Proteome and allergenome of the European house dust mite Dermatophagoides pteronyssinus. PLoS ONE, 14 (5). e0216171. ISSN 1932-6203

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Abstract

The European house dust mite Dermatophagoides pteronyssinus is of significant medical importance as it is a major elicitor of allergic illnesses. In this analysis we have undertaken comprehensive bioinformatic and proteomic examination of Dermatophagoides pteronyssinus airmid, identified 12,530 predicted proteins and validated the expression of 4,002 proteins. Examination of homology between predicted proteins and allergens from other species revealed as much as 2.6% of the D. pteronyssinus airmid proteins may cause an allergenic response. Many of the potential allergens have evidence for expression (n = 259) and excretion (n = 161) making them interesting targets for future allergen studies. Comparative proteomic analysis of mite body and spent growth medium facilitated qualitative assessment of mite group allergen localisation. Protein extracts from house dust contain a substantial number of uncharacterised D. pteronyssinus proteins in addition to known and putative allergens. Novel D. pteronyssinus proteins were identified to be highly abundant both in house dust and laboratory cultures and included numerous carbohydrate active enzymes that may be involved in cuticle remodelling, bacteriophagy or mycophagy. These data may have clinical applications in the development of allergen-specific immunotherapy that mimic natural exposure. Using a phylogenomic approach utilising a supermatrix and supertree methodologies we also show that D. pteronyssinus is more closely related to Euroglyphus maynei than Dermatophagoides farinae.

Item Type:	Article
Additional Information:	Citation: Waldron R, McGowan J, Gordon N, McCarthy C, Mitchell EB, Fitzpatrick DA (2019) Proteome and allergenome of the European house dust mite Dermatophagoides pteronyssinus. PLoS ONE 14(5): e0216171. https://doi.org/10.1371/journal.pone.0216171 . © 2019 Waldron et al. This is an open access article distributed under the terms of the Creative Commons Attribution License https://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Keywords:	Proteome; allergenome; European house dust mite; Dermatophagoides pteronyssinus;
Academic Unit:	Faculty of Science and Engineering > Biology Faculty of Science and Engineering > Research Institutes > Human Health Institute
Item ID:	11050
Identification Number:	https://doi.org/10.1371/journal.pone.0216171
Depositing User:	David Fitzpatrick
Date Deposited:	17 Sep 2019 14:34
Journal or Publication Title:	PLoS ONE
Publisher:	Public Library of Science
Refereed:	Yes
Funders:	Irish Research Council (IRC), Science Foundation Ireland (SFI)
URI:	https://journals.plos.org
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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