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    Activation of Neutrophils via IP3 Pathway Following Exposure to Demodex-Associated Bacterial Proteins


    McMahon, Fred and Banville, Nessa and Bergin, David and Smedman, Christian and Paulie, Staffan and Reeves, Emer P. and Kavanagh, Kevin (2016) Activation of Neutrophils via IP3 Pathway Following Exposure to Demodex-Associated Bacterial Proteins. Inflammation, 39 (1). pp. 425-433. ISSN 1573-2576

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    Abstract

    Rosacea is a chronic inflammatory condition that predominantly affects the skin of the face. Sera from rosacea patients display elevated reactivity to proteins from a bacterium (Bacillus oleronius) originally isolated from a Demodex mite from a rosacea patient suggesting a possible role for bacteria in the induction and persistence of this condition. This work investigated the ability of B. oleronius proteins to activate neutrophils and demonstrated activation via the IP3 pathway. Activated neutrophils displayed increased levels of IP1 production, F-actin formation, chemotaxis, and production of the pro-inflammatory cytokines IL-1β and IL-6 following stimulation by pure and crude B. oleronius protein preparations (2 μg/ml), respectively. In addition, neutrophils exposed to pure and crude B. oleronius proteins (2 μg/ml) demonstrated increased release of internally stored calcium (Ca2+), a hallmark of the IP3 pathway of neutrophil activation. Neutrophils play a significant role in the inflammation associated with rosacea, and this work demonstrates how B. oleronius proteins can induce neutrophil recruitment and activation.

    Item Type: Article
    Additional Information: Cite as: McMahon, F., Banville, N., Bergin, D.A. et al. Inflammation (2016) 39: 425. https://doi.org/10.1007/s10753-015-0264-4
    Keywords: Bacillus; Demodex; inflammation; neutrophils; rosacea;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 11100
    Identification Number: https://doi.org/10.1007/s10753-015-0264-4
    Depositing User: Dr. Kevin Kavanagh
    Date Deposited: 25 Sep 2019 15:42
    Journal or Publication Title: Inflammation
    Publisher: Springer US
    Refereed: Yes
    URI:

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